Antidiabetic pyrrolecarboxylic acids

ABSTRACT

Certain pyrrolecarboxylic and pyrroleacetic acid derivatives substituted on the pyrrole ring with thioether groups, acyl groups, phenyl, substituted phenyl, phenoxy, substituted phenoxy, benzyl or halo and optionally substituted on the pyrrole nitrogen with alkyl, and the pharmaceutically acceptable salts thereof, are useful in lowering the blood glucose levels of hyperglycemic animals.

CROSS REFERENCE TO RELATED APPLICATIONS

This is a division of application Ser. No. 256,933 filed Apr. 23, 1981,now U.S. Pat. No. 4,351,843 issued Sept. 29, 1982, which in turn is adivision of application Ser. No. 128,199 filed Mar. 7, 1980, now U.S.Pat. No. 4,282,242 issued Aug. 4, 1981.

BACKGROUND OF THE INVENTION

In spite of the early discovery of insulin and its subsequentwide-spread use in the treatment of diabetes, and the later discoveryand use of sulfonylureas (e.g. chlorpropamide, tolbutamide,acetohexamide, tolazamide) and biguanides (e.g. phenformin) as oralhypoglycemic agents, the treatment of diabetes remains less thansatisfactory. The use of insulin, necessary in a high percentage ofdiabetics where available synthetic hypoglycemic agents are noteffective, requires multiple daily, usually self, injection.Determination of the proper dosage of insulin requires frequentestimations of the sugar in the urine or in the blood. Theadministration of an excessive dose of insulin causes hypoglycemia, witheffects ranging from mild abnormalities in blood glucose to coma, oreven death. Where effective, synthetic hypoglycemic agents are preferredover insulin, being more convenient to administer and less prone tocause severe hypoglycemic reactions. However, the clinically availablehypoglycemics are fraught with other toxic manifestations which limittheir use. In any event, where one of these agents may fail in anindividual case, another may succeed. The need for additionalhypoglycemic agents, which may be less toxic or succeed where othersfail, is clearly evident.

In addition to the hypoglycemic agents cited above, a variety of othercompounds have been reported to possess this type of activity, including5-methylpyrazole-3-carboxylic acid [Smith, et al., J. Med. Chem. 8, 350(1965)], 3-methylisoxazole-5-carboxylic acid [Dulin and Gerritsen, Proc.Soc. Expt. Biol. Med. 121, 777 (1966)],1-methyl-4-(5-methyl-3-pyrazolyl)pyridinium iodide [Dulin et al., Proc.Soc. Expt. Biol. Med. 118, 499 (1965)],1-methyl-4-(3-methyl-5-isoxazolyl)pyridinium chloride [Bauer et al., J.Med. Chem. 11, 984 (1968)], 2-carboxypyrazine [Dulin and Gerritsen,Metab. Clin. Exp. 18, 214 (1969)], a series ofhexahydroindeno(1,2-c)pyrroles and dialkylaminomethylindans [Lahiri andPathak, J. Pharm. Sci. 57, 1013 (1968)], methylenecyclopropylacetic acidand 4-pentenoic acid [Corredor et al., Proc. Nat. Acad. Sci. U.S. 58,2299 (1967)].

A few of the compounds of the present invention have been previouslydisclosed. 4-Chloropyrrole-2-carboxylic acid, a compound of no knownprior utility, has been described by Fringuelli et al. [Tetrahedron 25,5815 (1969)]. The benzoyl (and substituted benzoyl) pyrrole carboxylicacids (IV, V, VI) of the present invention are generally disclosed byBelgian Pat. No. 870,910 as having uricosuric activity and thus usefulin the treatment of gout and related pathological conditions. Certaincompounds isomeric to those of the present invention have also beendisclosed: 5-chloropyrrole-2-carboxylic acid [Fringuelli et al., loc.cit.; no known utility], 4-benzoylpyrrole-2-carboxylic acid [Groves etal., Can. J. Chem. 49, 2427 (1971); Sanchez et al., Carb. Res. 3, 486(1967); generally disclosed to have uricosuric activity by Belgian Pat.No. 870,910; not active as hypoglycemic agents in the present case],3-benzoylpyrrole-2-carboxylic acid [Kahn et al., Tetrahedron 22, 2095(1966); generally disclosed to have uricosuric activity by Belgian Pat.No. 870,910], 5-phenylpyrrole-2-carboxylic acid [Blicke et al., J. Am.Chem. Soc. 66, 1675 (1944); no known utility] and2-phenylpyrrole-3-carboxylic acid [Kondo and Suzuki, J. Pharm. Soc.Japan No. 544, 501 (1927); Chem. Abstr. 21, 3362; no known utility].

In some cases, methyl or ethyl esters or other homologs of the compoundsof the present invention are known: methyl4-benzoylpyrrole-3-carboxylate [Groves et al., Can. J. Chem. 51, 1089(1973); no known utility], methyl 4-chloropyrrole-2-carboxylate[Fringuelli et al., loc. cit.; no known utility], and5-benzoyl-1-methylpyrrole-2-acetic acids [including tolmetin, thep-methylbenzoyl analog; Carson et al., J. Med. Chem. 14, 646 (1971);which compounds are reported to have anti-inflammatory activity].

SUMMARY OF THE INVENTION

It has now been found that compounds selected from the group consistingof:

(a) a first subgroup consisting of thioethers of pyrrole carboxylicacids of structure I, II or III: ##STR1## wherein n is an integer ofvalue 0 or 1, A is selected from the group consisting of hydrogen and(C₁ -C₂)alkyl and R is selected from the group consisting of (C₁-C₅)alkyl; benzyl; phenyl; phenyl monosubstituted in the 2-, 3- or4-position with methyl, methoxy, chloro, fluoro or trifluoromethyl;2,5-, 2,4- or 3,4-dichlorophenyl; and 2,4,5-trichlorophenyl;

(b) a second subgroup consisting of acylated pyrrole carboxylic acids ofstructure IV, V or VI: ##STR2## wherein A is selected from the groupconsisting of hydrogen and (C₁ -C₂)alkyl and R¹ is selected from thegroup consisting of benzyl; cyclohexyl; phenyl; phenyl monosubstitutedin the 2-, 3-, or 4-position with methyl, phenyl or chloro;2,5-dichlorophenyl; and 3,5-dimethoxyphenyl;

(c) a third subgroup consisting of monosubstituted pyrrole carboxylicacids of structure VIII or IX: ##STR3## wherein A is selected from thegroup consisting of hydrogen and (C₁ -C₂)alkyl; R⁴ is selected from thegroup consisting of benzyl, phenyl, chloro, 4-chlorophenyl and4-chlorophenoxy; and R³ is selected from the group consisting of benzyland chloro;

(d) a fourth group of disubstituted pyrrole carboxylic acids selectedfrom the group consisting of:

4,5-bis(4-methoxyphenylthio)pyrrole-3-carboxylic acid;

1-methyl-4,5-bis(phenylthio)pyrrole-2-carboxylic acid;

4-methyl-5-benzoylpyrrole-3-carboxylic acid;

4-phenyl-5-benzoylpyrrole-3-carboxylic acid;

4-bromo-5-benzoylpyrrole-2-carboxylic acid;

5-bromo-4-benzoylpyrrole-2-carboxylic acid; and

1-methyl-4,5-dibromopyrrole-2-carboxylic acid;

and the pharmaceutically-acceptable cationic salts thereof, whenadministered in appropriate amount, orally or parenterally, to rodentswill lower the level of blood glucose, projecting clinical use of thesecompounds to reduce the blood levels of glucose in hyperglycemicmammals, including man, to acceptable levels.

By the term "pharmaceutically-acceptable cationic salts" is intendedsalts such as the alkali metal salts, e.g., sodium and potassium;alkaline earth metal salts such as calcium and magnesium; aluminumsalts; ammonium salts; and salts with organic bases, e.g., amines suchas benzathine (N,N'-dibenzylethylenediamine), choline, diethanolamine,ethylenediamine, meglumine (N-methylglucamine), benethamine(N-benzylphenethylamine), diethylamine, piperazine and tromethamine(2-amino-2-hydroxymethyl-1,3-propanediol).

Because they possess a high level of activity, compounds of specialvalue in this invention are those of structure I wherein n is O, A ishydrogen, and R is n-butyl, phenyl, 2-, 3- or 4-chlorophenyl, 2,5- or3,4-dichlorophenyl, 2,4,5-trichlorophenyl or 4-fluorophenyl; that ofstructure I wherein n is 1, A is hydrogen and R is phenyl; those ofstructure III wherein n is O, A is hydrogen and R is phenyl or4-chlorophenyl; those of structure IV wherein A is hydrogen and R¹ iscyclohexyl, phenyl, 4-chlorophenyl or 4-phenylphenyl; that of structureIV wherein A is methyl and R¹ is phenyl; those of structure V wherein Ais hydrogen or methyl and R¹ is phenyl; those of structure VIII whereinA is hydrogen and R³ is benzyl or chloro; those of structure IX whereinA is hydrogen and R⁴ is phenyl, 4-chlorophenyl, 4-chlorophenoxy orchloro, and 1-methyl-4,5-bis(phenylthio)pyrrole-2-carboxylic acid.Because they possess an exceptionally high level of activity, compoundsof greatest value in this invention are those of structure I wherein nis O, A is hydrogen and R is n-butyl, phenyl, 2- or 3-chlorophenyl, 2,5-or 3,4-dichlorophenyl, 2,4,5-trichlorophenyl or 4-fluorophenyl; those ofstructure IV wherein A is methyl and R¹ is phenyl or A is hydrogen andR¹ is 4-phenylphenyl; that of structure VIII wherein A is hydrogen andR³ is chloro and those of structure IX wherein A is hydrogen and R⁴ is4-chlorophenyl or 4-chlorophenoxy.

DETAILED DESCRIPTION OF THE INVENTION

The pyrrolecarboxylic acids of the present invention are prepared usinga variety of synthetic methods, depending on the substituent and theposition of the substituent desired.

The thioethers of structure I (A═H; n═O) are generally prepared byreaction of a lower alkyl pyrrole-3-carboxylate ester with theappropriate sulfenyl chloride (sulfenylation) followed by acid or basecatalyzed hydrolysis of the resulting ester. The sulfenylation is aFriedel-Crafts type reaction. The sulfenyl halides, being highlyreactive, do not require the addition of a catalyst, although thereaction is self-catalyzed by the hydrogen chloride produced in thereaction. The reaction is conveniently carried out by mixing thereagents in an inert solvent such as an aromatic hydrocarbon (e.g.benzene, toluene), a halogenated aromatic or aliphatic hydrocarbon (e.g.chlorobenzene, methylene chloride, ethylene chloride, chloroform), or anether (tetrahydrofuran, 1,2-dimethoxyethane). As a matter of safety,since this reaction is in some cases exothermic, the sulfenyl chloride,as a solution or neat, is conveniently added dropwise to a solution ofthe furan-2-carboxylate at reduced temperature. The reaction is thenallowed to proceed at room temperature until substantially complete(about 1-16 hours). Temperature is not critical, and can besubstantially above or below ambient (e.g. 0°-50° C.); at lowertemperatures, the reaction time is extended beyond the 16 hour range ifnecessary. At the higher part of the temperature range, shorter reactiontimes (e.g. 15 minutes to 2 hours) are employed. The thioether estersare isolated by evaporation of solvent, followed by standardrecrystallization or chromatographic procedures. If desired, crude estercan be hydrolyzed, leaving purification to the final stage of thesynthesis. Hydrolysis of the resulting esters is readily carried outunder a variety of conditions, employing acid or base catalysis, wellknown in the chemical art. Conveniently, the hydrolysis is carried outin a mixture of aqueous sodium hydroxide and either methanol or ethanol,by heating for 1-4 hours on a steam bath under reflux or in an openflask. Product is isolated by evaporation of any remaining alcohol,acidification, and filtration or extraction into an organic solvent suchas ethyl acetate and evaporation to dryness. Further purification, whendesired, is by standard recrystallization or chromatographic techniques.

When thioethers of the structure I (A═CH₃ or C₂ H₅ ; n═O) are desired,it is convenient to alkylate the intermediate esters, prior tohydrolysis as described immediately above. Methylation or ethylation isconveniently carried out by first converting the5-substituted-pyrrole-2-carboxylate ester to the sodium salt by reactionwith sodium hydride in an inert solvent (i.e. one which will not itselfreact with sodium hydride, e.g. tetrahydrofuran, dimethoxyethane,benzene), followed by reaction with an alkylating agent (e.g.,dimethylsulfate, methyl iodide, methyl bromide, ethyl iodide, ethylchloride, etc.). Formation of the sodium salt is generally carried outat room temperature in the presence of excess sodium hydride for 1 hour,more or less. Subsequent alkylation is carried out, usually in the samesolvent, by addition of excess alkylating agent and reaction at 0°-50°C. for 1 to 24 hours, conveniently ambient temperature for about 16hours.

The sulfenyl chlorides required as starting materials are readilyavailable by the reaction of the appropriate mercaptan withN-chlorosuccinimide or chlorine in an inert solvent (carbontetrachloride, chloroform, methylene chloride, benzene, tetrahydrofuran,1,2-dimethoxyethane, etc.) at 0°-50° C. until reaction is substantiallycomplete (2-48 hours)--conveniently at ambient temperature for about 16hours. Methyl or other lower alkyl pyrrole-3-carboxylates areconveniently prepared by the condensation of tosylmethyl isocyanide withmethyl or other alkyl acrylate, in a solvent such as tetrahydrofuranwith sodium hydride as the source of basic catalyst.

The thioethers of structure II (n═O, A═H) are generally prepared bysulfenylation of pyrrole (under conditions described above for thesulfenylation of alkyl pyrrole-3-carboxylates, yielding in this case2-sulfenylpyrroles) followed by trichloroacetylation at the other alphaposition (Friedel-Crafts conditions similar to the initialsulfenylation) to yield intermediate 5-sulfenyl-2-trichloroacetylderivatives, and finally hydrolysis (conditions identical with thosedescribed above for ester hydrolyses) to yield the desired5-sulfenylpyrrole-2-carboxylic acids. When 1-methyl or ethyl derivatives(II, n═O, A═CH₃ or C₂ H₅) are desired, they can be obtained bydialkylation of the acids (II, n═O, A═H) under the alkylation conditionsdescribed above, followed by hydrolysis of the resulting ester, again asdescribed above.

The thioethers of structure III (n═O, A═H) are generally prepared by thesulfenylation of pyrrole-2-carboxylic acid. Conditions are like thesulfenylation conditions described above, except that a reaction periodup to five times longer is employed. The now acidic products areconveniently isolated by replacement of the solvent with awater-immiscible solvent (if necessary), extraction of the product intosodium hydroxide, acidification, filtration or extraction into anorganic solvent such as ethyl acetate and evaporation to dryness. Ifnecessary, the product is purified by standard recrystallizationtechniques or chromatography. When the 1-alkyl derivatives encompassedby structure II are desired, they are prepared from the acids by themethods described in the paragraphs above.

When compounds of the structures I, II, or III, wherein n=1, aredesired, they can be prepared by sequential hydride reduction of theappropriate acid or ester, to pyrrolylmethyl alcohols, conversion of thealcohol to the corresponding tosylate, mesylate, etc. and then to thenitrile. Hydrolysis of the nitriles produces the desired acids. Thehydride reductions can be carried out with a variety of reagents,generally under mild conditions. The most common commercially availablehydride reducing agents are diborane, lithium aluminum hydride, lithiumborohydride, and sodium borohydride. The latter can be activated byaddition of lithium chloride or aluminum chloride. Also commerciallyavailable is a less reactive derivative of lithium aluminum hydride soldunder the tradename "Red-al," which is a 70% solution ofbis(2-methoxyethoxy)aluminum hydride in benzene, and lithium aluminumhydride as a 50% suspension in oil, which is more readily handled thanlithium aluminum hydride itself. The reduction of carboxylic acids andesters requires a strong hydride reducing agent such as diborane,lithium aluminum hydride or sodium borohydride activated with aluminumchloride. It is essential that the solvent for such a hydride reductionbe aprotic and free of reducible groups (carbonyl function of any type,nitrile, nitro, aliphatic halogen, sulfonate, etc.). The preferredsolvents are ethers such as tetrahydrofuran, dioxane,1,2-dimethoxyethane, bis(2-methoxyethyl)ether , etc. Temperature andreaction time are not critical, usually being in the range 0°-100° C.for up to 24 hours. For reduction of esters the same reagents as thoseemployed for reduction of acids can be employed. Lithium borohydridealone can also be employed, but more vigorous conditions (e.g. refluxingtetrahydrofuran) are required. Also well-suited for the reduction ofesters is Red-al (discussed above). Suitable solvents for use withRed-al are toluene, benzene, diethylether, tetrahydrofuran,dimethoxyethane, etc. Temperature and reaction times are as discussedimmediately above. The intermediate tosylates, mesylates, etc., arereadily obtained by reaction of tosyl chloride, mesyl chloride, etc.with the alcohol in an inert solvent (such as those defined above forsulfenylations) over a wide temperature range (e.g. -50° to 80° C.) inthe presence of a basic catalyst, preferrably a tertiary amine such astriethylamine. The reaction is rapid, being complete in a matter ofminutes at room temperature. As an alternative, the alcohols can bereacted with reagents such as dry halogen halide, or phosphorous halidesto yield the corresponding halide, which can be substituted for thetosylate, mesylate, etc., in the next step. The tosylate, mesylate,etc., can be isolated by standard methods, or used directly in the nextstep without isolation. Replacement of mesylate (or other group) iseffected by cyanide anion, usually in the form of the potassium orsodium salt. A wide variety of solvents are suitable for this reaction,including water, alcohols, ketones, ethers, halogenated hydrocarbons,acetonitrile, dimethylformamide, etc., or miscible combinations thereof.The only requirements are that the solvent be inert towards reactantsand product, that the reactants have some degree of solubility, and thatthe solvent be less acidic than hydrogen cyanide so as to maintain theanionic form thereof. The temperature employed for this reaction is notcritical (e.g. 0°-120° C.). It should be high enough to provide areasonable rate, but not so high as to lead to undue decomposition. Asis well known in the art, rate will vary with the nature of the groupdisplaced, the structure of the substrate, the solvent, the temperatureand the concentration of the reactants. To maximize yields, the reactiontime should be such that the reaction is nearly complete (e.g. >95%conversion when equivalent amounts of mesylate and cyanide areemployed). These reactions are readily monitored by thin layerchromatography, employing one of a variety of commercially availablesilica gel plates containing an ultraviolet indicator. Suitable eluantsare ethyl acetate/hexane/methanol mixtures with about 5% added aceticacid. The proportion of these solvents is varied with the polarity ofthe reactant and product, a practice well-known in the art. For most ofthe reactions of this type, an eluant consisting of 5 parts of ethylacetate and 1 part of hexane together with 5% acetic acid is wellsuited. For the more polar compounds the proportion of ethyl acetate isincreased (e.g. 1 ethyl acetate, 1 hexane). The final step, hydrolysisof nitrile to acid, is carried out under conditions identical to thosedescribed above for the hydrolysis of alkyl esters.

In the case of compounds of the structure I, II and III, wherein n=1 andA═CH₃ or C₂ H₅, they can also be prepared by alkylation of the acids ofstructure I, II or III, wherein n=1 and A═H, and hydrolysis of theresulting ester, using methods described above. Alkylation can also becarried out at various precursor stages, e.g. on alcohol or nitrile.

Thioethers of the structure II (n=1, C═CH₃ or C₂ H₅) are alternativelyprepared by sulfenylation of 1-alkyl-2-pyrrolylacetonitrile, followed byhydrolysis, both reactions being carried out as described above. Furthervariation in the sequence of steps for preparing compounds I, II and III(A═CH₃ or C₂ H₅) will be evident to those skilled in the art.

5-Acylpyrrole-3-carboxylic acids of structure IV, wherein A═H areprepared by Friedel-Crafts type acylation of a loweralkylpyrrole-3-carboxylate, followed by hydrolysis of the resultingester. The acylation is generally carried out using an acid halide,usually the acid chloride, as the acylating agent. The acylationconditions (solvent, temperature, time) are identical to those describedabove for sulfenylations, except that an added Lewis acid catalyst isgenerally employed. A convenient catalyst is stannic chloride, but awide variety of other catalysts (e.g. aluminum chloride) are also usefulfor this purpose.

Acid halides required in the above syntheses, when not availablecommercially, are readily available by standard methods from thecorresponding acids, e.g. reaction of thienyl chloride with acids inmethylene chloride (optionally with a trace of dimethylformamide ascatalyst) is but one of many convenient ways of preparing acid chloridesfrom carboxylic acids.

Alternatively, 5-acylpyrrole-3-carboxylic acids are prepared fromalpha-haloketones, employing the following route: ##STR4##

The initial reaction of alpha-haloketone with imidazo[3,4-a]pyridine iscarried out under conditions identical to those described above for thereaction of cyanide with mesylate esters, in this case the solvent beingsufficiently non-acidic that the amine group is not protonated. Sincethe reactants/products are relatively polar, a relatively polar thinlayer chromatographic system is used to monitor this reaction--e.g.ethyl acetate/5% acetic acid as eluant. Condensation of the resultingsalt with a lower alkyl propiolate (e.g. R² ═CH₃ or C₂ H₅) affords thelower alkyl 1-(2-pyridylmethyl)-5-acylpyrrole-3-carboxylate. The latterreaction is facile in the presence of a weak base catalyst, such aspotassium carbonate, in a polar, aprotic solvent such asdimethylformamide. The reaction is carried out at a temperature between-10° C. and 50° C., conveniently ambient temperature, from 30 minutes toup to several days, depending upon the temperature selected. Thereaction is conveniently monitored by thin layer chromatography, as inthe preceding step. The 1-(2-pyridylmethyl) group is removed byreductive means (e.g. selenium dioxide in wet dioxane at 60°-120° C.,for 4-60 hours depending upon temperature; temperatures above theboiling point of dioxane are achieved by running the reaction underpressure). Finally, the ester is hydrolyzed by methods described aboveto yield the desired acid.

The imidazo[3,4-a]pyridine required for the above syntheses is readilyprepared by formylation of 2-aminomethylpyridine followed by cyclizationin the presence of excess phosphorus oxychloride. The necessaryalpha-haloketones are available commercially, in the literature or byliterature methods.

1-Alkyl derivatives of the structure IV (A═CH₃ or C₂ H₅) are preparedfrom the corresponding esters via the two step alkylation-hydrolysisprocedures described above.

5-Acylpyrrole-2-carboxylic acids (V, A═H) are prepared by two stageFriedel-Crafts acylation of alkyl pyrrole-2-carboxylates and hydrolysis,both reactions carried out according to procedures described above. Thecorresponding 1-alkyl derivatives (V, A═CH₃ or C₂ H₅) are prepared fromthe intermediate esters via the alkylation-hydrolysis procedure alsodescribed above.

5-Chloropyrrole-3-carboxylic acid (VIII, R³ ═Cl, A═H) is prepared bychlorination of lower alkyl pyrrole-3-carboxylate and hydrolysis of theresulting ester. When the corresponding 1-alkyl derivatives (VIII, R³═Cl, A═CH₃ or C₂ H₅) are desired, the intermediate esters are alkylatedprior to hydrolyses. Chlorination is conveniently carried out withexcess tert.-butyl hypochlorite as reagent in a solvent such asmethylene chloride. Alkylations and hydrolyses are carried out asdescribed above.

5-Benzylpyrrole-3-carboxylic acid (VIII, R³ ═C₆ H₅ CH₂, A═H) and1-alkyl-5-benzylpyrrole-3-carboxylic acids (VIII, R³ ═C₆ H₅ CH₂, A═CH₃or C₂ H₅) are conveniently prepared by reduction of the corresponding5-benzoylpyrrole-3-carboxylic acids (IV, R¹ ═C₆ H₅). The use of excesshydrazine and potassium hydroxide at elevated temperature (e.g.100°-150° C.) in a solvent such as ethylene glycol are conditionswell-suited for this purpose. The corresponding4-benzylpyrrole-2-carboxylic acid (IX, R⁴ ═C₆ H₅ CH₂, A═H) is preparedin the same manner from known 4-benzoylpyrrole-2-carboxylic acid.1-Alkyl derivatives of IX are prepared by methods described above.

The compounds of structure IX wherein R⁴ is 4-chlorophenoxy or4-chlorophenyl are prepared by the following route: ##STR5## Theappropriately substituted acetaldehyde di(lower alkyl)acetal (e.g. R²═CH₃ or C₂ H₅) is reacted with dimethylformamide and phosphorousoxychloride and then with dimethylamine to yield the intermediate3-dimethylamino acrylaldehyde, which in turn is reacted with a loweralkyl glycinate (e.g. R² ═CH₃ or C₂ H₅) to yield the pyrrole precursor.The cyclization is carried out by simple heating of the startingmaterial in ethylene glycol. The lower alkyl ester (e.g. R² ═CH₃ or C₂H₅) is simultaneously converted to the glycol ester. Alkylations andhydrolyses are carried out as described above. Compounds of thestructure IX wherein R⁴ is phenyl or benzyl can also be obtained bysubstitution of the appropriate acetal as starting material. Alternativesynthesis of the benzyl compounds are described above. Alternativesynthesis of the phenyl compounds is by hydrogenolysis of thecorresponding 4-chlorophenyl derivatives (e.g. hydrogenation overpalladium-on-carbon in ethanol/triethylamine).

The disubstitutedpyrrole carboxylic acids of this invention are preparedaccording to the methods detailed in the specific examples providedbelow.

The pharmaceutically-acceptable cationic salts of the compounds of thepresent invention are readily prepared by reacting the acid forms withan appropriate base, usually one equivalent, in a co-solvent. Typicalbases are sodium hydroxide, sodium methoxide, sodium ethoxide, sodiumhydride, potassium methoxide, magnesium hydroxide, calcium hydroxide,benzathine, choline, diethanolamine, ethylenediamine, meglumine,benethamine, diethylamine, piperazine and tromethamine. The salt isisolated by concentration to dryness, or by addition of a non-solvent.In some cases, salts can be prepared by mixing a solution of the acidwith a solution of a different salt of the cation (e.g. sodiumethylhexanoate, magnesium oleate), employing a solvent in which thedesired cationic salt precipitates, or can be otherwise isolated byconcentration and/or addition of a non-solvent.

The pyrrolecarboxylic acids of the present invention are readily adaptedto clinical use as antidiabetic agents. The hypoglycemic activityrequired for this clinical use is well illustrated by the test procedurewhich follows.

Intact male albino rats, each weighing approximately 200 grams are theexperimental test animals employed for such purposes. The test animalsare fasted approximately 18-24 hours. The rats are weighed, numbered,and recorded in groups of five or six as needed. Each animal is thendosed with glucose (usually one gram per kilogram) intra-peritoneally,and then either saline (controls) or compound. The treated animals aregiven the pyrrolecarboxylic acid to be tested at a dosage of 100 mg./kg.or less; in each instance, the drug is suspended or dissolved in anaqueous system, and the doses are administered orally or parenterally.Blood glucose is measured over a period of 3 house in both control andtreated groups. The results obtained are expressed in terms of thepercentage decrease in the blood glucose value of treated animals fromthe control value. Those compounds which decrease the blood glucose by20% or better are considered to have high activity; while those whichdecrease it by 30% or better are considered to have exceptionally highactivity. In this connection, it is significant to note that the resultsobtained show that the compounds of the present invention exhibit ahypoglycemic effect which is comparable to that afforded by knownclinically useful anti-diabetics in this field.

The pyrrolecarboxylic acids of the present invention are clinicallyadministered to diabetic mammals, including man, via either the oral orthe perenteral route. Administration by the oral route is preferred,being more convenient and avoiding the possible pain and irritation ofinjection. However, in circumstances where the patient cannot swallowthe medication, or absorption following oral administration is impaired,as by disease or other abnormality, it is essential that the drug beadministered parenterally. By either route, the dosage is in the rangeof about 0.10 to about 50 mg./kg. body weight of the subject per day,preferably about 0.10 to about 10 mg./kg. body weight per dayadministered singly or as a divided dose. However, the optimum dosagefor the individual subject being treated will be determined by theperson responsible for treatment, generally smaller doses beingadministered initially and thereafter increments made to determine themost suitable dosage. This will vary according to the particularcompound employed and with the subject being treated.

The compounds can be used in pharmceutical preparations containing thecompound, or a pharmaceutically-acceptable acid salt thereof, incombination with a pharmaceutically-acceptable carrier or diluent.Suitable pharmaceutically-acceptable carriers include inert solidfillers or diluents and sterile aqueous or organic solutions. The activecompound will be present in such pharmaceutical compositions in amountsufficient to provide the desired dosage amount in the range describedabove. Thus, for oral administration the compounds can be combined witha suitable solid or liquid carrier or diluent to form capsules, tablets,powders, syrups, solutions, suspensions and the like. The pharmaceuticalcompositions may, if desired, contain additional components such asflavorants, sweeteners, excipients and the like. For parenteraladministration the compounds can be combined with sterile aqueous ororganic media to form injectable solutions or suspensions. For example,solutions in sesame or peanut oil, aqueous propylene glycol and the likemay be used, as well as aqueous solutions of water-solublepharmaceutically-acceptable salts of the compounds. The injectablesolutions prepared in this manner can then be administeredintravenously, intraperitoneally, subcutaneously or intramuscularly,with intramuscular administration being preferred in man.

The present invention is illustrated by the following examples. However,it should be understood that the invention is not limited to thespecific details of these examples.

EXAMPLE 1 Methyl Pyrrole-3-Carboxylate

Sodium hydride (57% dispersion in oil, 10.5 g., 0.25 mole) was placed ina flame dried flask and washed twice with dry benzene. Drytetrahydrofuran (400 ml.) was added to the flask and the resultingslurry stirred under nitrogen. A mixture of methyl acrylate (11.3 ml.,0.128 moles) and tosylmethyl isocyanide (25 g., 0.128 moles) in 120 ml.of tetrahydrofuran was then added dropwise over 30 minutes. The reactionwas exothermic and the reaction mixture refluxed during this process.After stirring for an additional hour (without external heating), thereaction mixture was cooled in an ice-water bath and water (approx. 120ml.) added dropwise until solution resulted. The solution was allowed towarm and then extracted three times with ether. The combined etherextracts were back-washed with water and then saturated brine, driedover anhydrous sodium sulfate, filtered and evaporated in vacuo to yieldmethyl pyrrole-3-carboxylate (6.4 g., m.p. 78°-81° C., m/e 125).

Substitution of ethyl, propyl, isopropyl, phenyl, or benzyl acrylate formethyl acrylate is used to produce the corresponding ethyl, propyl,isopropyl, phenyl, or benzyl pyrrole-3-carboxylates.

EXAMPLE 2 Benzenesulfenyl Chloride

Under a nitrogen atmosphere, N-chlorosuccinimide (16.3 g., 0.22 mole)was slurried in 125 ml. of methylene chloride. While stirring at roomtemperature, benzenethiol (13.2 g., 0.12 mole) was added; 2 ml. initialaddition to start reflux and the remainder at a rate to maintain reflux(approx. 10 min.). The clear solution which resulted was then stirred atroom temperature for 30 minutes. A small amount of precipitate whichformed was removed by filtration. The filtrate, assumed to contain thetheoretical quantity of benzenesulfenyl chloride (17.3 g., 0.12 mole),was used immediately and directly in the next step. Alternatively,benzenesulfenyl chloride was isolated by evaporation to an oil prior toits further use.

EXAMPLE 3 Methyl 5-phenylthiopyrrole-3-carboxylate

Methyl pyrrole-3-carboxylate (15 g., 0.12 mole) was dissolved in 200 ml.of methylene chloride under nitrogen and cooled in an ice-water bath.Benzenesulfenyl chloride (approx 17.3 g., 0.12 moles) in approximately125 ml. of methylene chloride (freshly prepared by the procedure ofExample 2) was added dropwise over to the stirred reaction mixture. Theice-water bath was removed and the reaction stirred for 1 hour at roomtemperature. A volume of ether equal to that of the reaction mixture wasadded and the mixture clarified by filtration. The filtrate was washedwith water and saturated brine, dried over anhydrous sodium sulfate,filtered and evaporated to an oil (26 g.). The oil was chromatographedon 300 g. of silica gel with ethyl acetate/hexane (7/1) as eluant.Fractions of 200 ml. volume were collected. Fractions 3 to 8 wereevaporated to yield methyl 5-phenylthiopyrrole-3-carboxylate (6.9 g.,m.p. 107°-109° C.)

The same procedure is employed to convert ethyl, propyl, isopropyl,phenyl or benzyl pyrrole 3-carboxylates to, respectively:

Ethyl 5-phenylthiopyrrole-3-carboxylate;

Propyl 5-phenylthiopyrrole-3-carboxylate;

Isopropyl 5-phenylthiopyrrole-3-carboxylate;

Phenyl 5-phenylthiopyrrole-3-carboxylate; and

Benzyl 5-phenylthiopyrrole-3-carboxylate.

EXAMPLE 4 5-Phenylthiopyrrole-3-carboxylic Acid

Methyl 5-phenylthiopyrrole-3-carboxylate (6.8 g.) was combined with 70ml. of 1N sodium hydroxide and 120 ml. of methanol and heated to refluxfor 3 hours. Methanol was evaporated, an equal volume of water added andimpurities extracted into ether. The aqueous phase was acidified withconc. hydrochloric acid, and precipitated5-phenylthiopyrrole-3-carboxylic acid (6.1 g., m.p. 145°-147° C., m/e219) recovered by filtration.

By the same procedure ethyl, propyl, isopropyl, phenyl and benzyl5-phenylthiopyrrole-3-carboxylates are converted to5-phenylthiopyrrole-3-carboxylic acid.

EXAMPLE 5 Methyl 1-Methyl-5-phenylthiopyrrole-3-carboxylate

5-phenylthiopyrrole-3-carboxylic acid (470 mg., 2 mmoles) was combinedwith sodium hydride (170 mg. of 57% in oil dispersion, 4 mmoles) and 50ml. of ether and stirred under nitrogen for 1 hour at room temperature.To the sodium salt thus formed, was added dimethylsulfate (0.4 ml., 4.2mmoles) and the reaction stirred for approximately 16 hours. Thin layerchromatography on silica gel with ethyl acetate-1/hexane-5/5% aceticacid indicated the reaction was complete. The reaction mixture wasconcentrated to dryness to yield methyl1-methyl-5-phenylthiopyrrole-3-carboxylate (sodium salt) used directlyin the next step.

The same product is also prepared by substituting an equivalent ofmethyl iodide for dimethylsulfate in an otherwise identical process.Substitution of an equivalent of ethyl iodide, in this process willproduce ethyl 1-ethyl-5-phenylthiopyrrole-3-carboxylate.

The same procedures are used to prepare 1-alkylpyrroles and alkyl1-alkylpyrrolecarboxylates from pyrrole and alkyl pyrrolecarboxylates,respectively, except that only one equivalent of sodium hydride and oneequivalent of the alkylating agent is employed.

EXAMPLE 6 1-Methyl-5-phenylthiopyrrole-3-carboxylic Acid

The entire crude methyl 1-methyl-5-phenylthiopyrrole-3-carboxylate fromExample 5 was taken up in 30 ml. of methanol and 15 ml. of 1N sodiumhydroxide and boiled for 2 hours. The aqueous residue was extracted withether to remove impurities and then acidified to precipitate1-methyl-5-phenylthiopyrrole-3-carboxylic acid (427 mg., m.p. 160°-162°C., m/e 233).

Analysis: Calcd. for C₁₂ H₁₁ O₂ N₂ S: C, 61.80; H, 4.75; N, 6.01. Found:C, 62.17; H, 4.73; N, 6.05.

By the same process the corresponding 1-ethyl derivative of Example 5 isconverted to 1-ethyl-5-phenylthiopyrrole-3-carboxylic acid.

EXAMPLE 7 o-Toluenesulfenyl Chloride

N-chlorosuccinimide (5.34 g., 40 mmoles) was slurried in 50 ml. ofbenzene and cooled in an ice-water bath. o-Toluenethiol (4.96 g., 40mmoles) in 50 ml. of benzene was added dropwise over 15 minutes. Thereaction was warmed to room temperature and stirred for approximately 16hours. The reaction mixture was filtered and o-toluenesulfenyl chloride(5.53 g.) obtained as an oil by evaporation in vacuo.

EXAMPLE 8 Methyl 5-(2-Methylphenylthio)pyrrole-3-carboxylate

Under a nitrogen atmosphere, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was dissolved in 50 ml. of methylene chloride. The stirredsolution was cooled in an ice-water bath and o-toluenesulfenyl chloride(3.16 g., 20 mmoles) was added dropwise. The reaction mixture was warmedto room temperature and stirred for 1 hour. Thin layer chromatography(silica gel, chloroform eluant) indicated virtually complete reaction atthis time. An equal volume of ether was added and a small amount ofinsolubles removed by filtration. Crude product (5 g.) was obtained byevaporation in vacuo to a gum. The latter was chromatographed on 150 g.of silica gel with 75 ml. fractions of the chloroform eluant collected.Fractions 10 to 13 were combined and evaporated in vacuo to yieldpartially purified product (1.69 g., m.p. 114°-119° C.).Recrystallization from methylene chloride/hexane gave purified methyl5-(2-methylphenylthio)pyrrole-3-carboxylate (1.1 g., m.p. 126°-128° C.).

EXAMPLE 9 5-(2-Methylphenylthio)pyrrole- 3-carboxylic Acid

Methyl 5-(2-methylphenylthio)pyrrole-3-carboxylate (1.1 g.) was combinedwith 20 ml. of 1N sodium hydroxide and 50 ml. of methanol and refluxedfor 2.5 hours, at which time thin layer chromatography withhexane-5/ethyl acetate-1/5% acetic acid as eluant indicated reaction tobe complete. Methanol was removed by evaporation, the aqueous residuewas diluted with approximately one volume of water, and extracted withether. The aqueous phase was acidified with 6N hydrochloric acid and theproduct extracted into ethyl acetate. The ethyl acetate was washed withsaturated brine, dried over anhydrous sodium sulfate, filtered, andconcentrated in vacuo to crude product (1.0 g.). Recrystallization frommethylene chloride/hexane gave purified5-(2-methylphenylthio)pyrrole-3-carboxylic acid (590 mg., m.p. 151°-153°C., m/e 233).

Analysis: Calcd. for C₁₂ H₁₁ O₂ NS: C, 61.77; H, 4.75; N, 6.0. Found: C,62.04; H, 4.75; N, 5.87.

EXAMPLE 10 p-Toluenesulfenyl Chloride

By the method of Example 7, 6.2 g. of p-toluenethiol (6.2 g., 50 mmoles)was reacted with N-chlorosuccinimide (6.67 g., 50 mmoles) in 100 ml. ofbenzene to yield p-toluenesulfenyl chloride (7.0 g.) as an oil.

By the same method m-toluenethiol [Tarbell and Fukushima, Org. Synthesis27, 81 (1947)] is converted to m-toluenesulfenyl chloride.

EXAMPLE 11 Methyl 5-(4-methylphenylthio)pyrrole-3-carboxylate

Methyl pyrrole-3-carboxylate (2.5 g., 20 mmoles) was dissolved in 50 ml.of methylene chloride. To the stirred solution there was added dropwisep-toluenesulfenyl chloride (2.92 g., 20 mmoles), with the temperaturemaintained below 30° C. by occasional cooling with an ice-water bath.The reaction was stirred for 60 minutes at room temperature. Thereaction mixture was concentrated in vacuo to an oil. The residue wastaken up in a mixture of isopropyl alcohol and hexane, a small amount ofgum removed by filtration and the filtrate reevaporated in vacuo to anoil (5.0 g.). The latter was chromatographed on 150 g. of silica gel,with 100 ml. fractions of the benzene eluant collected. Fractions 10 to19 were combined to yield the desired product (1.41 g.) contaminatedwith an impurity showing a mass spectral peak at 281. The latter wasremoved by recrystallization from methylene chloride/hexane to yieldpurified methyl 5-(4-methylphenylthio)pyrrole-3-carboxylate (1.14 g.,m.p. 118°-121° C., m/e 247).

By the method of this Example and Examples 3 and 8, methylpyrrole-3-carboxylate is reacted with m-toluenesulfenyl chloride toyield methyl 5-(3-methylphenylthio)-pyrrole-3-carboxylate.

EXAMPLE 12 5-(4-Methylphenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(4-methylphenylthio)pyrrole-3-carboxylate (700 mg.) wasrefluxed with 15 ml. of 1N sodium hydroxide and 30 ml. of methanol for2.5 hours. The methanol was removed by evaporation and the aqueousresidue cooled to room temperature and extracted with ether. The aqueouslayer was acidified and the product extracted multiply with ethylacetate. The ethyl acetate extracts were combined, washed with saturatedbrine, dried over anhydrous sodium sulfate, filtered, and evaporated toan oil, which crystallized on standing (700 mg.). Recrystallization frommethylene chloride/hexane gave purified5-(4-methylphenylthio)pyrrole-3-carboxylic acid (432 mg., m.p. 155°-157°C., m/e 233).

Analysis: Calcd. for C₁₂ H₁₁ O₂ NS.0.125 H₂ O: C, 61.21; H, 4.78; N,5.95. Found: C, 61.36; H, 4.67; N, 5.46.

By the same procedure the other ester of Example 11 is hydrolyzed to:

5-(3-Methylphenylthio)pyrrole-3-carboxylic acid.

EXAMPLE 13 o-Chlorobenzenesulfenyl Chloride

Under nitrogen, N-chlorosuccinimide (5.34 g., 40 mmoles) was slurried in50 ml. of carbon tetrachloride and the stirred mixture cooled in anice-water bath. o-Chlorothiophenol (5.76 g., 40 mmoles), dissolved in 25ml. of carbon tetrachloride, was added dropwise. The ice-water bath wasremoved and the reaction mixture stirred at room temperature forapproximately 16 hours. The reaction was filtered, evaporated to an oil,taken up in hexane, refiltered and reconcentrated to yieldo-chlorobenzenesulfenyl chloride (6.8 g., oil).

EXAMPLE 14 Methyl 2-(2-Chlorophenylthio)pyrrole-3-carboxylate

Under a nitrogen atmosphere, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was dissolved in 50 ml. of methylene chloride and cooled in anice-water bath. To the stirred solution o-chlorosulfenyl chloride (3.56g., 20 mmoles) was added dropwise. The ice-water bath was removed andthe reaction was stirred at room temperature for 1 hour. An equal volumeof ether was added to the reaction mixture, which was filtered and crudeproduct isolated by evaporation of the filtrate to an oil (4.5 g.). Theoil was crystallized from methylene chloride and hexane to yieldpurified methyl 5-(2-chlorophenylthio)pyrrole-3-carboxylate (2.0 g.,m.p. 124°-130° C.).

EXAMPLE 15 5-(2-Chlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(2-chlorophenylthio)pyrrole-3-carboxylate (2.0 g.) was combinedwith 20 ml. of methanol and 20 ml. of 1N sodium hydroxide and refluxedfor 2 hours. The methanol was allowed to evaporate and the aqueousresidue diluted with approximately one volume of water, acidified withhydrochloric acid and the product extracted multiply into ethyl acetate.The ethyl acetate extracts were combined, washed with saturated brine,dried over anhydrous sodium sulfate, filtered and concentrated in vacuoto a gum. The gum was crystallized by trituration with hexane and crudeproduct (1.0 g., m.p. 160°-166° C.) recovered by filtration.Recrystallization from methylene chloride/hexane gave purified5-(2-chlorophenylthio)pyrrole-3-carboxylic acid (m.p. 174°-176° C., m/e253).

Analysis: Calcd for C₁₁ H₈ O₂ NClS.0.25 H₂ O C, 51.06; H, 3.29; N, 5.41.Found: C, 51.09; H, 3.27; N, 5.47.

EXAMPLE 16 m-Chlorobenzenesulfenyl Chloride

By the same method as Example 13, m-chlorothiophenol (5.76 g., 40mmoles) was converted to m-chlorobenzenesulfenyl chloride (6.09 g. ofoil).

EXAMPLE 17 Methyl 5-(3-Chlorophenylthio)pyrrole-3-carboxylate

By the procedure of Example 14, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was reacted with m-chlorobenzenesulfenyl chloride to yield crudeproduct as an oil (5.0 g.). The crude was chromatographed on 250 g. ofsilica gel, with 100 ml. fractions of the methylene chloride eluantcollected. Fractions 17 to 30 were combined and evaporated to yieldpurified methyl 5-(3-chlorophenylthio)pyrrole-3-carboxylate (1.8 g.,m.p. 90°-95° C., m/e 267) which crystallized on standing.

EXAMPLE 18 5-(3-Chlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(3-chlorophenylthio)pyrrole-3-carboxylate (1.5 g.) was refluxedwith 10 ml. of 1N sodium hydroxide and 20 ml. of methanol, at which timethin layer chromatography (silica gel with hexane-5/ethyl acetate-1/5%acetic acid as eluant) indicated hydrolysis was complete. The methanolwas allowed to evaporate. The aqueous residue was diluted withapproximately one volume of water and extracted twice with ether. Theaqueous phase was acidified with 6N hydrochloric acid and productextracted multiply into ethyl acetate. The combined ethyl acetateextracts were back-washed with water and then with saturated brine,dried over anhydrous sodium sulfate and evaporated to an oil.Trituration of the oil with hexane gave5-(3-chlorophenylthio)pyrrole-3-carboxylic acid (1.32 g., m.p. 134°-137°C., m/e 253).

Analysis: Calcd. for C₁₁ H₈ O₂ NClS: C, 52.07; H, 3.17; N, 5.52. Found:C, 52.27; H, 3.27; N, 5.52.

EXAMPLE 19 p-Chlorobenzenesulfenyl Chloride

Following the method of Harpp and Mathiaparnam [J. Org. Chem. 37, 1372(1972)], 14.4 g. of p-chlorothiophenol was converted to 16 g. ofp-chlorobenzenesulfenyl chloride (oil).

Alternatively, p-chlorosulfenyl chloride is prepared by the procedure ofExample 13.

EXAMPLE 20 Methyl 5-(4-chlorophenylthio)pyrrole-3-carboxylate

Methyl pyrrole-3-carboxylate (2.5 g., 20 mmole) was dissolved in 35 ml.of methylene chloride. p-Chlorobenzenesulfenyl chloride (4.0 g., 22mmoles) was added dropwise over approximately 2 minutes, and thereaction mixture was stirred at room temperature for 1 hour. Two volumesof ether were added, the mixture extracted twice with 20 ml. portions ofwater and the organic layer evaporated in vacuo. The resulting oil waschromatographed on 100 g. of silica gel with approximately 25 ml.fractions of the ethyl acetate-1/hexane-7 eluant collected. Fractions 19to 36 were combined and concentrated to yield methyl5-(4-chlorophenylthio)pyrrole-3-carboxylate (1.4 g., m.p. 122°-124° C.).

EXAMPLE 21 5-(4-Chlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(4-chlorophenylthio)pyrrole-3-carboxylate (1.4 g.) was combinedwith 20 ml. of methanol and 20 ml. of 1N sodium hydroxide and heated ona steam bath for 2 hours. The reaction was cooled, acidified with conc.hydrochloric acid. and the crude product which precipitated, collectedby filtration. Two recrystallizations from acetone/hexane affordedpurified 5-(4-chlorophenylthio)pyrrole-3-carboxylic acid (740 mg., m.p.171°-173° C.).

Analysis: Calcd for C₁₁ H₈ O₂ SCl: C, 52.07; H, 3.18; N, 5.52. Found: C,52.20; H, 3.17; N, 5.52.

EXAMPLE 22 2,5-Dichlorobenzenesulfenyl Chloride

The procedure of Example 13 was repeated, reacting a slurry ofN-chlorosuccinimide (3.72 g., 27.9 mmoles) in 50 ml. of carbontetrachloride with 2,5-dichlorothiophenol (5.0 g., 27.9 mmoles) in 25ml. of carbon tetrachloride. 2,5-Dichlorobenzenesulfenyl chloride (5.1g.) was isolated as an oil.

EXAMPLE 23 Methyl 5-(2,5-Dichlorophenylthio)pyrrole-3-carboxylate

Under a nitrogen atmosphere, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was dissolved in 50 ml. of methylene chloride and cooled withstirring in an ice-water bath. 2,5-Dichlorobenzenesulfenyl chloride(4.24 g., 20 mmoles) was added dropwise. The bath was removed and thereaction stirred for 1 hour at room temperature, at which time thinlayer chromatography (silica gel with ethyl acetate-1/hexane-5/5% aceticacid as eluant) indicated that virtually all of the starting pyrroleester had been consumed. An equal volume of ether was added, the mixturefiltered and the filtrate evaporated in vacuo to a partially crystallinegum (5.2 g.). The latter was recrystallized from methylenechloride/hexane to yield purified methyl5-(2,5-dichlorophenylthio)pyrrole-3-carboxylate (2.68 g., m.p. 162°-165°C.).

EXAMPLE 24 5-(3,5-Dichlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(3,5-dichlorophenylthio)pyrrole-3-carboxylate (2.5 g.) wascombined with 25 ml. of methanol and 25 ml. of 1N sodium hydroxide andrefluxed for 3 hours. The methanol was allowed to evaporate, the aqueousresidue diluted with approximately one volume of water and washed twicewith ether. The aqueous phase was acidified with conc. hydrochloric acidand the product extracted into ethyl acetate. The two ethyl acetateextracts were combined, washed with saturated brine, dried overanhydrous sodium sulfate, filtered and evaporated in vacuo to crudeproduct (1.84 g., m.p. 169°-172° C.). Recrystallization from chloroformafforded purified 5-(3,5-dichlorophenylthio)pyrrole-3-carboxylic acid(1.3 g., m.p. 175°-177° C.).

Analysis: Calcd for C₁₁ H₇ O₂ NCl₂ S: C, 45.84; H, 2.44; N, 4.86. Found:C, 45.69; H, 2.52; N, 4.85.

EXAMPLE 25 2,4-Dichlorobenzenesulfenyl Chloride

Following the procedure of Example 13, 2,4-dichlorothiophenol (7.16 g.,40 mmoles; Preparation 1) was converted to 2,4-dichlorobenzenesulfenylchloride (7.5 g., oil).

EXAMPLE 26 Methyl 5-(2,4-Dichlorophenylthio)pyrrole-3-carboxylate

Under nitrogen, methyl pyrrole-3-carboxylate (3.75 g.) was dissolved in100 ml. of methylene chloride and cooled in an ice-water bath. To thecold, stirred solution, 2,4-dichlorobenzenesulfenyl chloride (6.39 g.,30 mmoles) was added dropwise. The bath was removed and the reactionstirred at room temperature for 1 hour. An equal volume of ether wasadded, the mixture filtered and the filtrate evaporated in vacuo tocrude product (9.0 g., oil). The oil was chromatographed on 300 g. ofsilica gel with 200 ml. fractions of the ethyl acetate-1/hexane-7 eluantcollected. Fractions 6 to 10 were combined and evaporated to yieldpurified methyl 5-(2,4-dichlorophenylthio)pyrrole-3-carboxylate (3.7 g.,solid, m/e 302).

EXAMPLE 27 2-(2,4-Dichlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(2,4-dichlorophenylthio)pyrrole-3-carboxylate (3.6 g.) wascombined with 40 ml. of methanol and 40 ml. of 1N sodium hydroxide andrefluxed for 2.5 hours. The methanol was allowed to evaporate, theaqueous residue diluted with approximately one volume of water andextracted with ether. The aqueous phase was acidified with conc.hydrochloric acid and the heavy oil which precipitated extracted intoethyl acetate. The ethyl acetate extracts were combined, washed withsaturated brine, dried over anhydrous sodium sulfate, filtered andevaporated in vacuo to crude product (3.4 g., m.p. 160°-164° C.).Recrystallization from methanol/water gave purified5-(2,4-dichlorophenylthio)pyrrole-3-carboxylic acid (2.3 g., m.p.166°-168° C.).

Analysis: Calcd. for C₁₁ H₇ O₂ NCl₂ S: C, 45.84; H, 2.44; N, 4.86.Found: C, 45.68; H, 2.77; N, 5.05.

EXAMPLE 28 3,4-Dichlorobenzenesulfenyl Chloride

By the procedure of Examples 13 and 22, 2,4-dichlorothiophenol (5 g.,279 mmoles) was converted to 3,4-dichlorobenzenesulfenyl chloride (5.0g., oil).

EXAMPLE 29 Methyl 5-(3,4-Dichlorophenylthio)pyrrole-3-carboxylate

By the procedure of Example 23, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was reacted with 3,4-dichlorobenzenesulfenyl chloride (4.24 g.,20 mmoles) to yield crude product (6.3 g., oil). The crude waschromatographed on 250 g. of silica gel, with 125 ml. fractions of theethyl acetate-1/hexane-5/5% acetic acid eluant collected. Fractions 2 to6 were combined and evaporated to dryness to afford purified methyl5-(3,4-dichlorophenylthio)pyrrole-3-carboxylate (2.76 g., m.p. 93°-96°C.).

EXAMPLE 30 5-(3,4-Dichlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(3,4-dichlorophenylthio)pyrrole-3-carboxylate (1.7 g.) wascombined with 20 ml. of methanol and 20 ml. of 1N sodium hydroxide andrefluxed for 3 hours. The methanol was allowed to evaporate, andapproximately one volume of water was added to the aqueous residue,which was then extracted twice with ether, cooled in an ice-water bath,acidified with conc. hydrochloric acid, and the product extracted intoethyl acetate. The three ethyl acetate extracts were combined, washedwith saturated brine, dried over anhydrous sodium sulfate, filtered andevaporated in vacuo to a gummy solid which was crystallized bytrituration with hexane and recovered by filtration (1.34 g., m.p.156°-159° C.). Recrystallization from chloroform gave purified5-(3,4-dichlorophenylthio)pyrrole-3-carboxylic acid (850 mg., m.p.159°-161° C.).

Analysis: Calcd for C₁₁ H₇ O₂ NCl₂ S: C, 45.84; H, 2.44; N, 4.86. Found:C, 45.36; H, 2.58; N, 4.79.

EXAMPLE 31 2,4,5-Trichlorobenzenesulfenyl Chloride

By the procedure of Example 13, 2,4,5-trichlorothiophenol (8.54 g., 40mmoles) was converted to 2,4,5-trichlorobenzenesulfenyl chloride (8.2g., oil).

EXAMPLE 32 Methyl 5-(2,4,5-Trichlorophenylthio)pyrrole-3-carboxylate

By the procedure of Example 23, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was reacted with 2,4,5-trichlorobenzenesulfenyl chloride (4.95g., 20 mmoles) to yield crude product (6.0 g.) as a foam. The crude waschromatographed on 300 g. of silica gel with 200 ml. fractions of theethyl acetate-1/hexane-7 eluant collected. Fractions 6 to 12 werecombined and evaporated to yield methyl5-(2,4,5-trichlorophenylthio)pyrrole-3-carboxylate (2.2 g., m.p.165°-175° C.).

EXAMPLE 33 5-(2,4,5-Trichlorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(2,4,5-trichlorophenylthio)pyrrole-3-carboxylate (2.1 g.) wascombined with 20 ml. of methanol and 20 ml. of 1N sodium hydroxide andheated to reflux. Additional 1N sodium hydroxide was added in an amountsufficient to obtain a solution. Reflux was continued for 2.5 hours, themethanol was then allowed to evaporate, and the aqueous residue wasdiluted with approximately one volume of water and extracted twice withether. The aqueous phase was cooled in an ice-water bath and acidifiedwith conc. hydrochloric acid. Crude product (1.65 g., m.p. 210°-215° C.)was recovered by filtration. Recrystallization from methanol/waterafforded purified 5-(2,4,5-trichlorophenylthio)pyrrole-3-carboxylic acid(1.01 g., m.p. 215°-217° C.).

Analysis: Calcd. for C₁₁ H₆ O₂ NCl₃ S: C, 40.95; H, 1.87; N, 4.34.Found: C, 40.56; H, 2.14; N, 4.16.

EXAMPLE 34 p-Fluorobenzenesulfenyl Chloride

Employing the procedure of Example 13, p-fluorothiophenol (5.0 g., 39mmoles) and N-chlorosuccinimide (5.2 g., 39 mmoles) were reacted to formp-fluorobenzenesulfenyl chloride (4.8 g., oil).

By the same procedure m-fluorothiophenol (Preparation 1) is converted tom-fluorobenzenesulfenyl chloride.

EXAMPLE 35 Methyl 5-(4-fluorophenylthio)pyrrole-3-carboxylate

By the procedure of Example 26, methyl pyrrole-3-carboxylate (2.5 g., 20mmoles) was reacted with p-fluorobenzenesulfenyl chloride (3.24 g., 20mmoles), yielding crude product (4.6 g.) as an oil. The crude waschromatographed on 250 g. of silica gel, collecting 125 ml. fractions ofthe ethyl acetate-1/hexane-5/5% acetic acid eluant. Fractions 5 to 7were combined and evaporated to yield purified methyl5-(4-fluorophenylthio)pyrrole-3-carboxylate (1.5 g., m.p. 100°-105° C.,m/e 251).

By the same procedure, m-fluorobenzenesulfenyl chloride is reacted withmethyl pyrrole-3-carboxylate to form methyl5-(3-fluorophenylthio)pyrrole-3-carboxylate.

EXAMPLE 36 5-(4-Fluorophenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(4-fluorophenylthio)pyrrole-3-carboxylate (1.5 g.) was combinedwith 10 ml. of methanol and 10 ml. of 1N sodium hydroxide and refluxedfor 2 hours. The methanol was allowed to evaporate, the aqueous residuewas diluted with approximately one volume of water and extracted twicewith ether. The aqueous phase was cooled in an ice-water bath andacidified to pH 2.0 with conc. hydrochloric acid. The gummy solid whichprecipitated was extracted into ethyl acetate. The three combined ethylacetate extracts were washed with saturated brine, dried over anhydroussodium sulfate, filtered and evaporated to an oil. Trituration withhexane gave crystalline 5-(4-fluorophenylthio)pyrrole-3-carboxylic acid(1.17 g., m.p. 116-118, m/e 237), recovered by filtration.

Analysis: Calcd. for C₁₁ H₈ O₂ NFS: C, 55.68; H, 3.39; N, 5.90. Found:C, 55.78; H, 3.56; N, 5.66.

By the same procedure, methyl5-(3-fluorophenylthio)pyrrole-3-carboxylate is hydrolyzed to5-(3-fluorophenylthio)pyrrole-3-carboxylic acid.

EXAMPLE 37 m-Trifluoromethylbenzenesulfenyl Chloride

The procedure of Example 13 was employed to convertm-trifluorothiophenol (7.12 g., 40 mmoles) to m-trifluorobenzenesulfenylchloride (6.77 g. of oil).

EXAMPLE 38 Methyl 5-(3-Trifluoromethylphenylthio)pyrrole-3-carboxylate

Following the procedure of Example 26, m-trifluorobenzenesulfenylchloride (6.36 g., 30 mmoles) was reacted with methylpyrrole-3-carboxylate to yield 9.0 g. of crude product as an oil. Theoil was chromatographed identically. Fractions 7 to 9 were combined andevaporated in vacuo to yield purified methyl5-(3-trifluoromethylphenylthio)pyrrole-3-carboxylate (3.04 g., m.p.110°-115° C., m/e 301).

EXAMPLE 39 5-(3-Trifluoromethylphenylthio)pyrrole-3-carboxylic Acid

Methyl 5-(3-trifluoromethylphenylthio)pyrrole-3-carboxylate (2.9 g.) wascombined with 30 ml. of methanol and 30 ml. of 1N sodium hydroxide andrefluxed for 2.5 hours. The methanol was allowed to evaporate and theaqueous residue washed twice with ether. The aqueous phase was acidifiedwith conc. hydrochloric acid and the crude product (2.3 g) whichprecipitated, was recovered by filtration. Recrystallization of thecrude from methanol/water gave purified5-(3-trifluoromethylphenylthio)pyrrole-3-carboxylic acid (1.47 g., m.p.138°-140° C.).

Analysis: Calcd. for C₁₂ H₈ O₂ NF₃ S: C, 50.17; H, 2.80; N, 4.87. Found:C, 49.95; H, 3.06; N, 4.79.

EXAMPLE 40 Pyrrole-3-carboxylic Acid

Methyl pyrrole-3-carboxylate (3 g., 24 mmoles) was combined with 40 ml.of methanol and 40 ml. of 1N sodium hydroxide and refluxed for 1 hour.The methanol was allowed to evaporate, the aqueous residue was dilutedwith approximately one volume of water and extracted with ether. Theaqueous phase was cooled in an ice-water bath and acidified with conc.hydrochloric acid. A small amount of insoluble material was removed byfiltration and the filtrate was extracted three times with ethylacetate. The combined ethyl acetate extracts were back-washed with waterand then with saturated brine, dried over anhydrous sodium sulfate,filtered and evaporated to a gummy solid. Trituration with hexane gavecrystalline pyrrole-3-carboxylic acid (1.8 g., m.p. 115°-120° C., m/e111), recovered by filtration.

EXAMPLE 41 p-Methoxybenzenesulfenyl Chloride

Following the procedure of King and Abikar [Can. J. Chem. 46, 9(1968),], 50 ml. of carbon tetrachloride was cooled to 0°-5° C. in anice-water bath and saturated with gaseous chlorine. This cold solutionwas titrated dropwise into a cold (0°-5° C.) solution ofp-methoxythiophenol (5 g.) in 25 ml. of carbon tetrachloride insufficient quantity to maintain a deep red color. The reaction wasevporated to an oil. Distillation gave purified p-methoxybenzenesulfenylchloride (3.59 g., b.p. 107° C./4 mm).

By the same procedure or by the procedure of Example 13,o-methoxythiophenol and m-methoxythiophenol are converted, respectively,to o-methoxybenzenesulfenyl chloride and m-methoxybenzenesulfenylchloride.

EXAMPLE 42 5-(4-Methoxyphenylthio)pyrrole-3-carboxylic Acid and4,5-bis(4-methoxyphenylthio)pyrrole-3-carboxylic Acid

Under a nitrogen atmosphere, pyrrole-3-carboxylic acid (1.93 g., 17mmoles) was dissolved in 50 ml. of tetrahydrofuran and cooled in anice-water bath. To the stirred cold solution, p-methoxysulfenyl chloride(3.0 g., 17 mmoles) was added dropwise. The bath was removed and thereaction was stirred for approximately 16 hours at room temperature. Anequal volume of ether was added, the mixture was filtered and thefiltrate evaporated in vacuo to yield an oil (4.5 g.). The oil waschromatographed on 250 g. of silica gel, with 200 ml. fractions of theethyl acetate-1/hexane-5/5% acetic acid eluant collected. Fractions 5 to7 were combined and evaporated to an oil, which was crystallized fromether/hexane to yield 4,5-bis(4-methoxyphenylthio)pyrrole-3-carboxylicacid (265 mg., m.p. 159°-162° C.).

Analysis: Calcd. for C₁₉ H₁₇ O₄ NS₂ : C, 58.89; H, 4.42; N, 3.61. Found:C, 58.44; H, 4.27; N, 3.73.

The ether/hexane mother liquor was evaporated to oil, crystallized bytrituration with hexane (472 mg.). Recrystallization of the latter frommethylene chloride gave 5-(4-methoxyphenylthio)pyrrole-3-carboxylic acid(200 mg., m.p. 144°-147° C.).

Analysis: Calcd. for C₁₂ H₁₁ O₃ NS: C, 57.81; H, 4.44; N, 5.61. Found:C, 57.54; H, 4.51; N, 5.46.

The latter product is also prepared by reaction ofp-methoxybenzenesulfenyl chloride with methyl pyrrole-3-carboxylate,using the procedures of Example 3, followed by hydrolysis according toExample 4. In this case the chromatography system of the present exampleis used to purify the product, if necessary.

The same two-step procedure of Examples 3 and 4 is used to converto-methoxybenzenesulfenyl chloride and m-methoxybenzenesulfenyl chlorideto, respectively:

5-(2-Methoxyphenylthio)pyrrole-3-carboxylic acid; and

5-(3-Methoxyphenylthio)pyrrole-3-carboxylic acid.

EXAMPLE 43 Ethylsulfenyl Chloride

Diethyl disulfide (610 mg., 5 mmoles) was dissolved in 10 ml. of carbontetrachloride. A solution of chlorine in carbon tetrachloride (3.5 ml.of concentration 100 mg./ml.) was added and the solution stirred for 10minutes at room temperature. The resulting solution, used directly insubsequent steps, was estimated to contain 10 mmoles of ethylsulfenylchloride.

EXAMPLE 14 Methyl 5-Ethylthiopyrrole-3-carboxylate

Methyl pyrrole-3-carboxylate (1 g., 8 mmoles) was dissolved in 10 ml. ofmethylene chloride and stirred under nitrogen. A solution ofethylsulfenyl chloride in carbon tetrachloride as prepared above(estimated 10 mmoles) was added, followed by stannic chloride (2.3 ml.,20 mmoles). The reaction mixture was stirred at room temperature for 2hours. A precipitate formed. A mixture of ice and water (approximately10 ml.) and ethyl acetate (20 ml.) was added to the reaction mixture,which was then added to 100 ml. of ether. The organic phase was washedtwice with 50 ml. of water, once with 50 ml. of 1N sodium hydroxide,twice more with 25 ml. portions of water and finally concentrated to anoil (1 g.). The reaction was repeated on 5.5 times this scale. In thiscase, the supernatant was decanted from the precipitate which had formedby the end of the reaction period. Water (100 ml.) and ether(approximately 300 ml.) was added to the supernatant. The organic phasewas washed with water and concentrated to an oil (1.3 g.). Theprecipitate was dissolved in 150 ml. of ethyl acetate and 100 ml. ofwater. The ethyl acetate layer was washed with 50 ml. of water, filteredand concentrated to dryness (4.8 g.). The three crude products abovewere combined (approximately 6.5 g.), dissolved in approximately 5 ml.of ethyl acetate and chromatographed on 400 ml. of silica gel, elutedwith ethyl acetate-1/hexane-6/5% acetic acid. The purification wasmonitored by thin layer chromatography (silica gel with the sameeluant). Middle cuts containing clean product (Rf 0.4) were concentratedto dryness and triturated with hexane to yield slightly gummy crystals(1.15 g.). Recrystallization of 444 mg. of crude from ether/hexane gavepurified methyl 5-ethylthiopyrrole-3-carboxylate (140 mg., m.p. 67°-70°C.). Additional crude product (approximately 300 mg.) was recovered byevaporation of the recrystallization mother liquor.

EXAMPLE 45 5-Ethylthiopyrrole-3-carboxylic Acid

Methyl 5-ethylthiopyrrole-3-carboxylic acid (100 mg.) was combined with5 ml. of ethanol and 5 ml. of 1N sodium hydroxide and heated on a steambath in an open flask for 1 hour. The aqueous residue was cooled to roomtemperature and extracted with 2 ml. of ether. The aqueous phase wasacidified with conc. hydrochloric acid to yield crystalline5-ethylthiopyrrole-3-carboxylic acid (38 mg., m.p. 110°-112° C., m/e171).

Analysis: Calcd. for C₇ H₉ O₂ NS: C, 49.11; H, 5.30; N, 8.18. Found: C,48.96; H, 5.21; N, 7.91.

EXAMPLE 46 Butylsulfenyl Chloride

Under nitrogen, dibutyl disulfide (3.56 g., 20 mmoles) was dissolved in40 ml. of carbon tetrachloride and the stirred solution cooled in anice-water bath. A solution of chlorine (1.4 g., 20 mmoles) in 14 ml. ofcarbon tetrachloride was added dropwise. The bath was removed and thesolution stirred for 10 minutes at room temperature. The solutionestimated to contain 4.96 g. (40 mmoles) of butylsulfenyl chloride wasused directly in subsequent steps.

By the same process, dimethyl disulfide [Hunter and Sorenson, J. Am.Chem. Soc. 54, 3364 (1932)], dipentyl disulfide [Miller et al., J. AmChem. Soc. 64, 2323 (1942)], and dicyclohexyl disulfide (Preparation 3)are converted respectively, to solutions of methylsulfenyl chloride,pentylsulfenyl chloride and cyclohexylsulfenyl chloride.

EXAMPLE 47 5-Butylthiopyrrole-3-carboxylic Acid

Under nitrogen, pyrrole 3-carboxylic acid (4.44 g., 40 mmoles) wasdissolved in 50 ml. of tetrahydrofuran and the solution cooled in anice-water bath. A solution of butylsulfenyl chloride (Example 46,estimated to contain 4.96 g., 40 mmoles) was added dropwise. The bathwas removed and stirring continued for 1 hour at room temperature. Anequal volume of ether was added, the mixture was filtered, and thefiltrate evaporated to an oil (8.3 g.). The oil was chromatographed on250 g. silica gel, with 100 ml. fractions of the ethylacetate-1/toluene-5/5% acetic acid eluant collected. Fraction 9 wasevaporated and triturated with carbon tetrachloride to yield crystalline5-butylthiopyrrole-3-carboxylic acid (185 mg., m/e 199). Fractions 7-8and 9-10 were combined, evaporated to gums, and rechromatographed insmaller columns, collecting 8 ml. fractions of the ethylacerate-1/heptane-2 eluant. An additional 114 mg. of5-butylthiopyrrole-3-carboxylic acid resulted.

Analysis: Calcd. for C₉ H₁₃ O₂ NS: C, 54.24; H, 6.57; N, 7.03. Found: C,54.74; H, 6.94; N, 6.98.

By the same procedure or by the procedures of Examples 44 and 45,methylsulfenyl chloride, pentylsulfenyl chloride, and cyclohexylsulfenylchloride are used to prepare 5-methylthiopyrrole-3-carboxylic acid,5-pentylthiopyrrole-3-carboxylic acid, and5-cyclohexylthiopyrrole-5-carboxylic acid.

EXAMPLE 48 Benzylsulfenyl Chloride

Following the procedure of Example 46, dibenzyl disulfide (4.92 g., 20mmoles) was converted to a solution estimated to contain 6.32 g. (40mmoles) of benzylsulfenyl chloride.

EXAMPLE 49 5-Benzylthiopyrrole-3-carboxylic Acid and4,5-bis-(Benzylthio)pyrrole-3-carboxylic Acid

Under nitrogen, pyrrole-3-carboxylic acid (4.44 g., 40 mmoles) wasdissolved in 50 ml. of tetrahydrofuran and cooled in an ice-water bath.A solution of benzylsulfenyl chloride (estimated 6.32 g., 40 mmoles) incarbon tetrachloride, freshly prepared by the method of Example 48, wasadded dropwise to the stirred solution. The bath was removed and thereaction stirred at room temperature for 1 hour. An equal volume ofether was added and the product extracted into 125 ml. of 1N sodiumhydroxide. The aqueous phase was back-washed with ether, acidified withconc. hydrochloric acid and the product extracted into ethyl acetate.The three ethyl acetate extracts were combined, back-washed with water,then twice with saturated brine, dried over anhydrous sodium sulfate andevaporated to an oil (8.0 g.). The oil was chromatographed on 300 g. ofsilica gel, with 200 ml. fractions of the ethyl acetate-1/toluene-5/5%acetic acid collected. Fractions 3 and 4 were combined and evaporated toa gummy solid (2.5 g.). The latter was triturated with hexane and thenwith ether to yield crystalline 4,5-bis-(benzylthio)pyrrole-3-carboxylicacid (298 mg., m.p. 160°-162° C., m/e 355).

Analysis: Calcd. for C₁₉ H₁₇ O₂ NS₂ : C, 64.19; H, 4.82; N, 3.94. Found:C, 64.33; H, 4.75; N, 4.34.

The mother liquor from the above crystalline product was evaported to agum (1.2 g.) and rechromatographed on 50 g. of silica gel, withcollection of 50 ml. fractions of the ethyl acetate-1/hexane-5/5% aceticacid eluant. Fractions 6 to 9 were combined, evaporated to a gummysolid, and crystallized from ether/hexane to yield5-benzylthiopyrrole-3-carboxylic acid (400 mg., m.p. 114°-117° C., m/e233).

Analysis: Calcd. for C₁₂ H₁₁ O₂ NS: C, 61.77; H, 4.75; N, 6.00. Found:C, 61.79; H, 4.83; N, 5.63.

EXAMPLE 50 1-(5-Phenylthio-3-pyrrolyl)methanol

Under nitrogen, 5-phenylthiopyrrole-3-carboxylic acid (1.1 g., 5 mmoles)was dissolved in 30 ml. of tetrahydrofuran. A 1M solution of borane intetrahydrofuran (10 ml., 10 mmoles) was added dropwise over 1 minute.The reaction was stirred for 13 minutes at room temperature, cooled inan ice-water bath and excess diborane decomposed by careful dropwiseaddition of 20 ml. of water. Ether (30 ml.) and 10 ml of 1N sodiumhydroxide was then added. The organic phase was separated, washed withwater and evaporated in vacuo to mixture of oil and crystals (0.8 g.).The reaction was repeated, except that stirring time was reduced to 10minutes, yielding 1.0 g. of crude product. The two crude products werecombined and chromatographed on approximately 200 g. of silica geleluted with ethyl acetate-1/hexane-1. Purified1-(5-phenylthio-3-pyrrolyl)methanol (800 mg., m.p. 69°-71° C., m/e 205)was isolated from the last fractions by evaporation, trituration withhexane and filtration.

By the same procedure, other 5-substituted pyrrole-3-carboxylic acidsprepared in the Examples above are converted to the corresponding1-(5-substituted-3-pyrrolyl)methanols, e.g.:

1-(1-Methyl-5-phenylthio-3-pyrrolyl)methanol;

1-[5-(2-Methylphenylthio)-3-pyrrolyl]methanol;

1-[5-(4-Methylphenylthio)-3-pyrrolyl]methanol;

1-[5-(2-Chlorophenylthio)-3-pyrrolyl]methanol;

1-[5-(3-Chlorophenylthio)-3-pyrrolyl]methanol;

1-[5-(4-Chlorophenylthio)-3-pyrrolyl]methanol;

1-[5-(2,5-Dichlorophenylthio)-3-pyrrolyl]methanol;

1-[5-(3,4-Dichlorophenylthio)-3-pyrrolyl]methanol;

1-[5-(2,4,5-Trichlorophenylthio-3-pyrrolyl]methanol;

1-[5-(4-Fluorophenylthio)-3-pyrrolyl]methanol;

1-[5-(3-Trifluoromethylphenylthio)-3-pyrrolyl]methanol;

1-[5-(4-Methoxyphenylthio)-3-pyrrolyl]methanol;

1-(5-Ethylthio-3-pyrrolyl)methanol;

1-(5-Butylthio-3-pyrrolyl)methanol; and

1-(5-Benzylthio-3-pyrrolyl)methanol.

EXAMPLE 51 1-(5-Phenylthio-3-pyrrolyl)methyl Mesylate

1-(5-Phenylthio-3-pyrrolyl)methanol (2.3 g., 11 mmoles) was dissolved in60 ml. of methylene chloride. Triethylamine (10 ml., 70 mmoles) wasadded and the solution was cooled to -40° to -45° C. With stirring,methanesulfonyl chloride (4 ml., 51 mmoles) was added dropwise,maintaining the temperature in the same range. The reaction was stirredfor 15 minutes at -40° to -45° C. and the cold solution of1-(5-phenylthio-3-pyrrolyl)methyl mesylate used directly in the nextstep.

By the same procedure, the other alcohols of Example 50 are converted tothe corresponding mesylate esters;

1-(1-Methyl-5-phenylthio-3-pyrrolyl)methyl mesylate;

1-[5-(2-Methylphenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(4-Methylphenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(2-chlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(3-Chlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(4-Chlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(2,5-Dichlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(3,4-Dichlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(2,4,5-Trichlorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(4-Fluorophenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(3-Trifluoromethylphenylthio)-3-pyrrolyl]methyl mesylate;

1-[5-(4-Methoxyphenylthio)-3-pyrrolyl]methyl mesylate;

1-(5-Ethylthio-3-pyrrolyl)methyl mesylate;

1-(5-Butylthio-3-pyrrolyl)methyl mesylate; and

1-(5-Benzylthio-3-pyrrolyl)methyl mesylate.

EXAMPLE 52 1-(5-Phenylthio-3-pyrrolyl)methyl Nitrile

Potassium cyanide (2 g.) was dissolved in 5 ml. of water and 30 ml.acetone added--resulting in two phases. The cold (-40° C.), methylenechloride solution of 1-(5-phenylthio-3-pyrrolyl)methyl mesylate ofExample 57 (estimated to contain 11 mmoles) was poured into thevigorously stirred, two-phase system. Stirring at room temperature wascontinued for 30 minutes. The reaction mixture was concentrated in vacuoto remove acetone and methylene chloride. Water (10 ml.) was added tothe aqueous residue, and the product was extracted into 25 ml. of ether.The ether was back-washed with 15 ml. of water and evaporated to an oil(200 mg.). The oil was chromatographed on approximately 75 ml. of silicagel, with ethyl acetate-1/hexane-2 as eluant. Evaporation of the initialfractions gave purified 1-(5-phenylthio-3-pyrrolyl)methyl nitrile (157mg., oil; pnmr/δ: methylene band at 3.50 ppm shifted from 4.35 ppm inthe precursor alcohol).

By the same procedure the other mesylate esters of Example 51 areconverted to:

1-(1-Methyl-5-phenylthio-3-pyrrolyl)methyl nitrile;

1-[5-(2-Methylphenylthio)-3-pyrrolyl)methyl nitrile;

1-[5-(4-Methylphenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(2-chlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(3-Chlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(4-chlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(2,5-Dichlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(3,4-Dichlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(2,4,5-Trichlorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(4-Fluorophenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(3-Trifluoromethylphenylthio)-3-pyrrolyl]methyl nitrile;

1-[5-(4-Methoxyphenylthio)-3-pyrrolyl]methyl nitrile;

1-(5-Ethylthio-3-pyrrolyl)methyl nitrile;

1-[5-Butylthio-3-pyrrolyl)methyl nitrile; and

1-[5-Benzylthio-3-pyrrolyl)methyl nitrile.

EXAMPLE 53 2-(5-Phenylthio-3-pyrrolyl)acetic Acid

1-(5-Phenylthio-3-pyrrolyl)methyl nitrile (3.2 g., crude, i.e. materialprepared by the method of Example 52 which had not been chromatographed)was combined with 75 ml. of ethanol and 50 ml. of 1N sodium hydroxideand refluxed for 4 hours. The condenser was removed and most of theethanol evaporated under a stream of nitrogen. The residual solution wasdiluted with 50 ml. of water and extracted with 50 ml. of ether. Theresulting three phases were separated. The upper (ether) phase wasdiscarded. The intermediate phase was diluted with 50 ml. of water,washed with ether and the ether discarded. Both the original loweraqueous phase and the second aqueous phase were acidified with conc.hydrochloric acid and in each case, product extracted into 25 ml. ofethyl acetate, the ethyl acetate backwashed twice with 15 ml. of waterand stirred to gummy solid (0.8 g. from the original lower phase; 1.9 g.from the intermediate phase). The crude products were combined andchromatographed on approximately 200 ml. of silica gel, eluted withethyl acetate-1/hexane-5/5% acetic acid. Evaporation of middle fractionsgave purified 2-(5-phenylthio-3-pyrrolyl)acetic acid. (1.50 g., m.p.125°-127° C.).

Analysis: Calcd. for C₁₂ H₁₁ O₂ NS: C, 61.80; H, 4.75; N, 6.01. Found:C, 61.75; H, 4.70; N, 6.04.

By the same procedure the other nitriles of Example 58 are converted to:

2-(1-Methyl-5-phenylthio-3-pyrrolyl)acetic acid;

2-[5-(2-Methylphenylthio)-3-pyrrolyl]acetic acid;

2-[5-(4-Methylphenylthio)-3-pyrrolyl]acetic acid;

2-[5-(2-Chlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(3-Chlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(4-Chlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(2,5-Dichlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(3,4-Dichlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(2,4,5-Trichlorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(4-Fluorophenylthio)-3-pyrrolyl]acetic acid;

2-[5-(3-Trifluoromethylphenylthio)-3-pyrrolyl]acetic acid;

2-[5-(4-Methoxyphenylthio)-3-pyrrolyl]acetic acid;

2-(5-Ethylthio-3-pyrrolyl)acetic acid;

2-(5-Butylthio-3-pyrrolyl)acetic acid; and

2-(5-Benzylthio-3-pyrrolyl)acetic acid.

EXAMPLE 54 2-Phenylthiopyrrole

Pyrrole (2.0 g., 30 mmoles) was dissolved in 20 ml. of ether.Benzenesulfenyl chloride (4.5 g., 30 mmoles) was added dropwise at sucha rate that the purple color of the reagent was discharged. A slightlyexothermic reaction was noted. After addition was complete (10 minutes),the reaction was evaporated to an oil (5.7 g.). The oil waschromatographed on approximately 180 ml. of silica gel, eluted withethyl acetate-1/hexane-7. Middle fractions, evaporated to dryness,afforded 2-phenylthiopyrrole (300 mg.).

Analysis: Calcd. for C₁₀ H₉ NS: C, 68.56; H, 5.18; N, 8.00. Found: C,68.15; H, 5.27; N, 7.83.

By the same procedure, the sulfenyl chlorides of the above examples arereacted with pyrrole or with a 1-alkylpyrrole (Example 5) to form thecorresponding 2-substituted pyrroles, e.g.:

1-Methyl-2-phenylthiopyrrole;

2-(2-Methylphenylthio)pyrrole;

2-(4-Methylphenylthio)pyrrole;

2-(2-Chlorophenylthio)pyrrole;

2-(3-Chlorophenylthio)pyrrole;

2-(4-Chlorophenylthio)pyrrole;

2-(2,5-Dichlorophenylthio)pyrrole;

2-(3,4-Dichlorophenylthio)pyrrole;

2-(2,4,5-Trichlorophenylthio)pyrrole;

2-(4-Fluorophenylthio)pyrrole;

2-(4-Methoxyphenylthio)pyrrole;

2-Ethylthiopyrrole;

2-Butylthiopyrrole; and

2-Benzylthiopyrrole.

EXAMPLE 55 2-Phenylthio-5-trichloroacetylpyrrole

2-Phenylthiopyrrole (1.75 g., 10 mmoles) was dissolved in 20 ml. ofmethylene chloride. Trichloroacetyl chloride (1.1 ml., 10 mmoles) wasadded and the reaction stirred for 3 hours at room temperature. Thereaction mixture was washed with 20 ml. of water and concentrated toyield 2-phenylthio-5-trichloroacetylpyrrole, used directly in the nextstep.

By this procedure the other 2-substituted pyrroles of Example 54 areconverted to:

1-Methyl-2-phenylthio-5-trichloroacetylpyrrole;

2-(2-Methylphenylthio)-5-trichloroacetylpyrrole;

2-(4-Methylphenylthio)-5-trichloroacetylpyrrole;

2-(2-Chlorophenylthio)-5-trichloroacetylpyrrole;

2-(3-Chlorophenylthio)-5-trichloroacetylpyrrole;

2-(4-Chlorophenylthio)-5-trichloroacetylpyrrole;

2-(2,5-Dichlorophenylthio)-5-trichloroacetylpyrrole;

2-(3,4-Dichlorophenylthio)-5-trichloroacetylpyrrole;

2-(2,4,5-Trichlorophenylthio)-5-trichloroacetylpyrrole;

2-(4-Fluorophenylthio)-5-trichloroacetylpyrrole;

2-(4-Methoxyphenylthio)-5-trichloroacetylpyrrole;

2-Ethylthio-5-trichloroacetylpyrrole;

2-Butylthio-5-trichloroacetylpyrrole; and

2-Benzylthio-5-trichloroacetylpyrrole.

EXAMPLE 56 5-Phenylthiopyrrole-2-carboxylic Acid

2-Phenylthiopyrrole-5-trichloroacetylpyrrole (estimated 10 mmoles, theentire product from Example 57) was combined with 25 ml. of 1N sodiumhydroxide and 60 ml of methanol and refluxed for one hour. The condenserwas removed and most of the methanol removed by boiling (approximately20 minutes). The reaction mixture was cooled to room temperature andby-products removed by filtration. The filtrate was extracted with 25ml. of ether, the aqueous phase acidified with conc. hydrochloric acidand the product extracted into 35 ml of ethyl acetate. The ethyl acetatephase was backwashed with water and concentrated to a solid (0.7 g.).Recrystallization from ether/hexane gave purified5-phenylthiopyrrole-2-carboxylic acid (475 mg., m.p. 129°-131° C., m/e219).

Analysis: Calcd. for C₁₁ H₉ O₂ NS: C, 60.27; H, 4.14; N, 6.39. Found: C,60.37; H, 4.08; N, 6.37.

A second crop of product was also obtained (190 mg., m.p. 126°-128° C.).

By the same procedure, the other substituted pyrroles of Example 55 areconverted to:

1-Methyl-5-phenylthiopyrrole-2-carboxylic acid;

5-(2-Methylphenylthio)pyrrole-2-carboxylic acid;

5-(4-Methylphenylthio)pyrrole-2-carboxylic acid;

5-(2-Chlorophenylthio)pyrrole-2-carboxylic acid;

5-(3-Chlorophenylthio)pyrrole-2-carboxylic acid;

5-(4-Chlorophenylthio)pyrrole-2-carboxylic acid;

5-(2,5-Dichlorophenylthio)pyrrole-2-carboxylic acid;

5-(3,4-Dichlorophenylthio)pyrrole-2-carboxylic acid;

5-(2,4,5-Trichlorophenylthio)pyrrole-2-carboxylic acid;

5-(4-Fluorophenylthio)pyrrole-2-carboxylic acid;

5-(4-Methoxyphenylthio)pyrrole-2-carboxylic acid;

5-Ethylthiopyrrole-2-carboxylic acid;

5-Butylthiopyrrole-2-carboxylic acid; and

5-Benzylthiopyrrole-2-carboxylic acid.

EXAMPLE 57 1-Methyl-5-phenylthio-2-pyrrolylmethyl Nitrile

Under nitrogen, 1-methyl-2-pyrrolylmethyl nitrile (12 g., 0.1 mole) wasdissolved in 250 ml. of methylene chloride and cooled with stirring inan ice-water bath. A solution of benzenesulfenyl chloride (estimated14.4 g., 0.1 mole) in 125 ml. of methylene chloride was added dropwise.The bath was removed and the reaction stirred at room temperature for 1hour. An equal volume of ether was added, the reaction extracted twicewith water, and the organic layer evaporated to an oil (23 g.). The oilwas chromatographed on 300 g. of silica gel, with 250 ml. fractions ofthe ethyl acetate-2/hexane-7 eluant collected. Fraction 2 was evaporatedto yield 1-methyl-5-phenylthio-2-pyrrolylmethyl nitrile (9.0 g., oil; Rf0.25 on thin layer chromatography on silica gel with ethylacetate-2/hexane-7 as eluant.

By the same procedure, the other sulfenyl chlorides of the aboveexamples are reacted with 2-pyrrolylmethyl nitrile,1-methyl-2-pyrrolylmethyl nitrile or other 1-alkyl-2-pyrrolylmethylnitriles as appropriate to yield 5-substituted-2-pyrrolylmethylnitriles, e.g.:

5-Phenylthio-2-pyrrolyl nitrile;

1-Ethyl-5-phenylthio-2-pyrrolyl nitrile;

5-(2-Methylphenylthio)-2-pyrrolyl nitrile;

5-(4-Methylphenylthio)-2-pyrrolyl nitrile;

5-(2-Chlorophenylthio)-2-pyrrolyl nitrile;

5-(3-Chlorophenylthio)-2-pyrrolyl nitrile;

5-(4-Chlorophenylthio)-2-pyrrolyl nitrile;

5-(2,5-Dichlorophenylthio)-2-pyrrolyl nitrile;

5-(3,4-Dichlorophenylthio)-2-pyrrolyl nitrile;

5-(2,4,5-Trichlorophenylthio)-2-pyrrolyl nitrile;

5-(4-Fluorophenylthio)-2-pyrrolyl nitrile;

5-(4-Methoxyphenylthio)-2-pyrrolyl nitrile;

5-Ethylthio-2-pyrrolyl nitrile;

5-Butylthio-2-pyrrolyl nitrile; and

5-Benzylthio-2-pyrrolyl nitrile.

The 2-pyrrolylmethyl nitriles required for the above syntheses, when notavailable commercially or in the literature, are derived frompyrrole-2-carboxylic acid by the procedures of Examples 50 to 52 and 5,i.e., diboran reduction, mesylate ester formation, conversion to nitrileand, if required, alkylation.

EXAMPLE 58 2-(1-Methyl-5-phenylthio-2-pyrrolyl)acetic Acid

1-Methyl-5-phenylthio-2-pyrrolylmethyl nitrile (2.0 g.) was combinedwith 20 ml. of 5N sodium hydroxide and 20 ml. of ethanol and refluxedfor 2 hours. The ethanol was allowed to evaporate and the aqueousresidue was diluted with approximately one volume water, washed twicewith ether, cooled in an ice-water bath, acidified with conc.hydrochloric acid and the product extracted into ethyl acetate. Thethree ethyl acetate extractions were combined, dried over anhydroussodium sulfate, and evaporated to crude product (1.5 g.).Recrystallization from ether/hexane afforded purified2-(1-methyl-5-phenylthio-2-pyrrolyl)acetic acid (770 mg., m.p. 94°-96°C., m/e 247).

Analysis: Calcd. for C₁₃ H₁₃ O₂ NS: C, 63.13; H, 5.29; N, 5.66. Found:C, 63.31; H, 5.33; N, 5.62.

By the same procedure, the other 5-substituted-2-pyrrolylmethyl nitrilesof Example 57 are converted to:

2-(5-Phenylthio-2-pyrrolyl)acetic acid;

2-(1-Ethyl-5-phenylthio-2-pyrrolyl)acetic acid;

2-[5-(2-Methylphenylthio)-2-pyrrolyl]acetic acid;

2-[5-(4-Methylphenylthio)-2-pyrrolyl]acetic acid;

2-[5-(2-Chlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(3-Chlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(4-Chlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(2,5-Dichlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(3,4-Dichlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(2,4,5-Trichlorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(4-Fluorophenylthio)-2-pyrrolyl]acetic acid;

2-[5-(4-Methoxyphenylthio)-2-pyrrolyl]acetic acid;

2-(5-Ethylthio-2-pyrrolyl)acetic acid;

2-(5-Butylthio-2-pyrrolyl)acetic acid; and

2-(5-Benzylthio-2-pyrrolyl)acetic acid.

EXAMPLE 59 4-Phenylthiopyrrole-2-carboxylic Acid

Pyrrole-2-carboxylic acid (5.5 g., 50 mmoles) was dissolved to theextent of about 90% by warming in 200 ml. of tetrahydrofuran. Thestirred, partial solution was cooled to -34° C., and freshly preparedbenzenesulfenyl chloride (7.5 g., 52 mmoles) added at this temperature.The reaction mixture was gradually warmed to room temperature overapproximately 2.5 hours and left to stir at room temperature forapproximately 64 hours. Insoluble material was removed by filtration.The filtrate was diluted with 200 ml. of ether and the product extractedinto 150 ml. of 1N sodium hydroxide. The aqueous phase was back-washedwith 75 ml. of ether, acidified with conc. hydrochloric acid and theproduct extracted into 75 ml. of ether. The ether was back-washed with50 ml. of water, concentrated to dryness (9.0 g.), the residue takenback up in 30 ml. of ether, insolubles (0.6 g., mostly startingmaterial) removed by filtration, and reconcentrated to crude product (8g.). The latter was chromatographed on approximately 350 g. of silicagel, with approximately 25 ml. fractions of the ethylacetate-1/hexane-7/5% acetic acid eluant collected. Fractions,concentrated to dryness, were monitored by thin layer chromatographyemploying the same eluant. Late fractions, including some containing alittle starting material, were combined and recrystallized fromether/hexane to yield purified 4-phenylthiopyrrole-2-carboxylic acid(0.34 g., m.p. 181°-183° C., m/e 219).

Analysis: Calcd. for C₁₁ H₉ O₂ NS: C, 60.27; H, 4.14; N, 6.39. Found: C,60.58; H, 4.24; N, 6.53.

EXAMPLE 60 4-(4-Chlorophenylthio)pyrrole-2-carboxylic Acid

Pyrrole-2-carboxylic acid (7.8 g., 70 mmoles) was for the most partdissolved in 350 ml. of tetrahydrofuran by stirring at room temperature.The partial solution was cooled with stirring to -40° C.p-Chlorobenzenesulfenyl chloride (13 g., 72 mmoles) was added at -40° C.and the reaction allowed to warm to room temperature gradually over a 3hour period. The reaction mixture was left to stand for approximately 64hours. The reaction mixture was diluted with 200 ml. of ether andextracted with 125 ml. of 1N sodium hydroxide, from which 5 g. of crudeproduct was isolated by acidification, extracted into ethyl acetate andconcentrated to dryness. Pure product was obtained by extraction of theabove tetrahydrofuran-ether phase with 50 ml. of water, acidification ofthe aqueous phase with conc. hydrochloric acid, extraction of productinto 50 ml. of ethyl acetate, back-wash with water and concentration ofthe ethyl acetate to dryness, yielding 5.7 g. of crude product. Two-foldrecrystallization of the latter from acetone-hexane afforded pure4-(4-chlorophenylthio)pyrrole-2-carboxylic acid (908 mg. in two crops;m.p. 218°-220° C., m/e 253).

Analysis: Calcd. for C₁₁ H₈ O₂ NSCl: C, 52.07; H, 3.18; N, 5.52. Found:C, 52.11; H, 3.32; N, 5.31. C, 52.13; H, 3.41; N, 5.25.

By the procedures of Examples 59 and 60, other sulfenyl chlorides, asprepared in the Examples above, are reacted with pyrrole-2-carboxylicacid to prepare the corresponding 4-substituted pyrrole-2-carboxylicacids, e.g.:

4-(2-Methylphenylthio)pyrrole-2-carboxylic acid;

4-(4-Methylphenylthio)pyrrole-2-carboxylic acid;

4-(2-Chlorophenylthio)pyrrole-2-carboxylic acid;

4-(3-Chlorophenylthio)pyrrole-2-carboxylic acid;

4-(2,5-Dichlorophenylthio)pyrrole-2-carboxylic acid;

4-(3,4-Dichlorophenylthio)pyrrole-2-carboxylic acid;

4-(2,4,5-Trichlorophenylthio)pyrrole-2-carboxylic acid;

4-(4-Fluorophenylthio)pyrrole-2-carboxylic acid;

4-(3-Trifluoromethylphenylthio)pyrrole-2-carboxylic acid;

4-(4-Methoxyphenylthio)pyrrole-2-carboxylic acid;

4-Ethylthiopyrrole-2-carboxylic acid;

4-Butylthiopyrrole-2-carboxylic acid; and

4-Benzylthiopyrrole-2-carboxylic acid.

The acids produced by the procedure of this Example are converted to thecorresponding 1-alkyl derivatives by application of the alkylationprocedure of Example 5 thereto, followed by the hydrolysis of theresulting 1-alkyl alkyl ester according to the procedure of Example 6.

The acids produced by the procedure of this Example are converted to thecorresponding 2-(4-substituted-2-pyrrolyl)acetic acids by applicationthereto of the diborane reduction, mesylate ester formation, conversionto nitrile and hydrolysis sequence of Examples 50 to 53.

EXAMPLE 61 1-Methyl-4,5-bis(phenylthio)pyrrole-2-carboxylic Acid

1-Methylpyrrole-2-carboxylic acid (5 g., 40 mmoles) was dissolved in 50ml. of methylene chloride. Benzenesulfenyl chloride (12 g., 80 mmoles)was added portionwise over approximately 2 minutes. The resultingreaction mixture was stirred for 4.5 hours at room temperature.1-Methyl-4,5-bis(phenylthio)pyrrole-2-carboxylic acid (1.5 g., m.p.172°-174° C., m/e 341) was recovered directly from the reaction mixture.A second crop was obtained from the mother liquor by diluting with 2volumes of ether, extraction of the product into 50 ml. of 1N sodiumhydroxide and acidification of the aqueous phase (0.70 g., m.p.172°-174° C.).

Analysis: Calcd. for C₁₈ H₁₅ NO₂ S₂ : C, 63.34; H, 4.43; N, 4.10. Found:C, 62.94; H, 4.35; N, 4.34.

By replacing 1-methylpyrrole-2-carboxylic acid with an equivalent amountof 1-ethylpyrrole-2-carboxylic acid, the procedure of this exampleproduces 1-ethyl-4,5-bis-(phenylthio)pyrrole-2-carboxylic acid.

EXAMPLE 62 Methyl 5-Benzoylpyrrole-3-carboxylate

Under nitrogen, methyl pyrrole-3-carboxylate (5 g., 40 mmoles) wascombined with benzoyl chloride (4.8 ml., 40 mmoles) and stannic chloride(9.2 ml. 80 mmoles) in 75 ml. of methylene chloride and stirred for 3hours at room temperature. Ice and water (50 ml.) was added slowly andstirring continued for an additional 15 minutes. Ether (150 ml.) wasthen added and, after equilibration, the phases separated. The upper,organic phase was washed sequentially with 50 ml. of water, 50 ml. of 1Nsodium hydroxide and 50 ml. of water, and evaporated to dryness to yieldcrude product (6.4 g. of solid). Recrystallization from acetonehexanegave purified methyl 5-benzoylpyrrole-3-carboxylate (4.1 g., Rf 0.4 onsilica gel thin layer chromatography eluted with ethylacetate-1/hexane-5/5% acetic acid).

Belgian patent 870,910 (published Mar. 29, 1979) also discloses, in ageneral fashion, that Friedel-Crafts acylation of pyrrole-3-carboxylatesyields 5-acylpyrrole-3-carboxylates. This patent specificallyexemplifies preparation of methyl1-methyl-5-(3-chlorobenzoyl)pyrrole-3-carboxylate (see Example 84below).

EXAMPLE 63 5-Benzoylpyrrole-3-carboxylic Acid

Methyl 5-benzoylpyrrole-3-carboxylate (7.5 g.) was combined with 100 ml.of 1N sodium hydroxide and 100 ml. of ethanol and boiled in an openflask for 1 hour, by which time most of the ethanol had evaporated. Theaqueous residue was diluted with 100 ml. of water and acidified withconc. hydrochloric acid to precipitate crystalline5-benzoylpyrrole-3-carboxylic acid [6.3 g., m.p. 289°-290° C. (dec.),m/e 215].

Belgian patent 870,910 (cited above) discloses preparation of analogousacids by hydrolysis of the corresponding ester.

EXAMPLE 64 Methyl 5-(2-Methylbenzoyl)pyrrole-3-carboxylate

Under nitrogen at room temperature, methyl pyrrole-3-carboxylate (2.5g., 20 mmoles) was combined with 75 ml. of methylene chloride. o-Toluoylchloride (3.09 g., 20 mmoles) in 25 ml. of methylene chloride was added,followed by stannic chloride (4.6 ml., 40 mmoles) added at a fastdropwise rate via a needle and syringe. Progress of the reaction wasfollowed by thin layer chromatography (silica gel with ethylacetate-1/hexane-5/5% acetic acid as eluant). After stirring at roomtemperature for 2.5 hours, the reaction was cooled with an ice-waterbath while 75 ml. of water was added dropwise. After addition wascomplete, the mixture was stirred for 15 minutes at room temperature,approximately an equal volume of ether was added, the organic layerseparated, washed in sequence with water, 1N sodium hydroxide, water andsaturated brine, dried over anhydrous sodium sulfate, filtered andevaporated to yield methyl 5-(2-methylbenzoyl)pyrrole-3-carboxylate.

EXAMPLE 65 5-(2-Methylbenzoyl)pyrrole-3-carboxylic Acid

Methyl 5-(2-methylbenzoyl)pyrrole-3-carboxylic acid (3.6 g.) wascombined with 40 ml. of methanol and 40 ml. of 1N sodium hydroxide,refluxed for 2.5 hours, the methanol evaporated, the aqueous residuediluted with approximately one volume of water and extracted with ether.The aqueous phase was cooled and acidified with conc. hydrochloric acidto yield crude product (4.0 g.), recovered by filtration.Recrystallization from methanol afforded purified5-(2-methylbenzoyl)pyrrole-3-carboxylic acid (2.2 g., m.p. 280°-282°C.).

Analysis: Calcd. for C₁₃ H₁₁ O₃ N: C, 68.11; H, 4.83; N, 6.11. Found: C,68.34; H, 4.97; N, 6.16.

EXAMPLE 66 Methyl 5-(4-Methylbenzoyl)pyrrole-3-carboxylate

Following the procedure of Example 64, p-toluoyl chloride (3.09 g., 20mmoles) and methyl pyrrole-3-carboxylate were reacted to form methyl5-(4-methylbenzoyl)pyrrole-3-carboxylate (2.9 g., m.p. 155°-159° C., m/e243).

By the same procedure, equivalent amounts of m-toluoyl chloride,o-fluorobenzoyl chloride, m-fluorobenzoyl chloride, p-fluorobenzoylchloride, o-chlorobenzoyl chloride, m-chlorobenzoyl chloride, andp-chlorobenzoyl chloride are reacted with methyl pyrrole-3-carboxylateto produce, respectively:

Methyl 5-(3-methylbenzoyl)pyrrole-3-carboxylate;

Methyl 5-(2-fluorobenzoyl)pyrrole-3-carboxylate;

Methyl 5-(3-fluorobenzoyl)pyrrole-3-carboxylate;

Methyl 5-(4-fluorobenzoyl)pyrrole-3-carboxylate;

Methyl 5-(2-chlorobenzoyl)pyrrole-3-carboxylate;

Methyl 5-(3-chlorobenzoyl)pyrrole-3-carboxylate;

Methyl 5-(4-chlorobenzoyl)pyrrole-3-carboxylate;

EXAMPLE 67 5-(4-Methylbenzoyl)pyrrole-3-carboxylic Acid

Methyl 5-(4-methylbenzoyl)pyrrole-3-carboxylate (2.7 g.) was refluxedfor 2 hours with 30 ml. of methanol and 30 ml. of sodium hydroxide, andthe product isolated by the procedure of Example 65. The crude product(3.23 g.) was recrystallized from methanol to produce purified5-(4-methylbenzoyl)pyrrole-3-carboxylic acid (1.32 g., m.p. 275°-277°C.).

Analysis: Calcd, for C₁₃ H₁₁ O₃ N: C, 68.11; H, 4.83; N, 6.11. Found: C,67.95; H, 4.95; N, 6.07.

By the same procedure, the other esters of Example 66 are converted to:

5-(3-Methylbenzoyl)pyrrole-3-carboxylic acid;

5-(2-Fluorobenzoyl)pyrrole-3-carboxylic acid;

5-(3-Fluorobenzoyl)pyrrole-3-carboxylic acid;

5-(4-Fluorobenzoyl)pyrrole-3-carboxylic acid;

5-(2-Chlorobenzoyl)pyrrole-3-carboxylic acid;

5-(3-Chlorobenzoyl)pyrrole-3-carboxylic acid; and

5-(4-Chlorobenzoyl)pyrrole-3-carboxylic acid.

EXAMPLE 68 2-(4-Chlorobenzoylmethyl)imidazo[3,4-a]pyridinium Bromide

Imidazo[3,4-a]pyridine (8.3 g., 70 mmoles) dissolved in 125 ml. of etherwas mixed with p-chlorophenacyl bromide (16.3 g., 70 mmoles) dissolvedin 50 ml. of tetrahydrofuran and stirred at room temperature for 10minutes at which time a gummy precipitate (approximately 2 g.) wasseparated by decantation. The decant was stirred for an additional 22hours at room temperature, by which time product had precipitatedheavily. Filtration with ether wash gave2-(4-chlorobenzoylmethyl)imidazo[3,4-a]pyridinium bromide (16.8 g., m.p.241°-243° C.).

By the same procedure, imidazo[3,4-a]pyridine is reacted with phenacylbromide, p-fluorophenacyl chloride, and p-methylphenacyl bromide toyield, respectively:

2-Benzoylmethylimidazo[3,4-a]pyridinium bromide;

2-(4-Fluorobenzoylmethylimidazo[3,4-a]pyridinium chloride; and

2-(4-Methylbenzoylmethylimidazo[3,4-a]pyridinum bromide.

EXAMPLE 69 Ethyl1-(2-Pyridylmethyl)-5-(4-chlorobenzoyl)pyrrole-3-carboxylateHydrochloride

2-(4-Chlorobenzoyl)imidazo[3,4-a]pyridinium bromide (10.5 g., 30 mmoles)was stirred with potassium carbonate (16.5 g., 120 mmoles) in 125 ml. ofdimethylformamide for 45 minutes at room temperature. The mixture wascooled to -10° C., and ethyl propiolate (4.65 ml., 35 mmoles) was added.The temperature rose to 0° C. The reaction mixture was then warmed toroom temperature, stirred for 4 hours, and filtered. The filtrate wasdiluted with 250 ml. of ether and extracted with two 250 ml. portions ofwater. The water extracts were combined and back-washed with 250 ml. ofether. The original organic layer and the ether back-wash were combined,dried over anhydrous sodium sulfate, and concentrated to an oil. The oilwas dissolved in 150 ml. of ether. Hexane (150 ml.) was added and aprecipitate removed by filtration. Addition of excess hydrogen chloridein ethyl acetate to the filtrate precipitated crystalline ethyl1-(2-pyridylmethyl)-2-(4-chlorobenzoyl)pyrrole- 4-carboxylatehydrochloride (5.1 g., m.p. 179°-182° C.).

By the same procedure, the other pyridinium halides of Example 68 areconverted to:

Ethyl 1-(2-pyridylmethyl)-5-(benzoyl)pyrrole-3-carboxylatehydrochloride.

Ethyl 1-(2-pyridylmethyl)-5-(4-fluorobenzoyl)pyrrole-3-carboxylatehydrochloride; and

Ethyl 1-(2-pyridylmethyl)-5-(4-methylbenzoyl)pyrrole-3-carboxylatehydrochloride.

EXAMPLE 70 Ethyl 5-(4-Chlorobenzoyl)pyrrole-3-carboxylate

Ethyl 1-(2-pyridylmethyl)-5-(4-chlorobenzoyl)pyrrole-3-carboxylatehydrochloride (4.05 g.) was combined with 150 ml. of dioxane, 5 ml. ofwater and 10 g. of selenium dioxide and refluxed for 18 hours. Thereaction mixture was filtered, the filtrate diluted with 150 ml. ofether and 150 ml. of water, and equilibrated. The organic phase wasseparated, back-washed with two 100 ml. portions of water, andconcentrated to yield solid ethyl5-(4-chlorobenzoyl)pyrrole-3-carboxylate (2.7 g., Rf 0.55 on silica gelthin layer chromatography with ethyl acetate-1/hexane-5/5% acetic acidas eluant).

By the same procedure, the other 1-(2-pyridylmethyl)pyrroles of Example69 are converted to:

Ethyl 5-benzoylpyrrole-3-carboxylate;

Ethyl 5-(4-fluorobenzoyl)pyrrole-3-carboxylate; and

Ethyl 5-(4-methylbenzoyl)pyrrole-3-carboxylate.

EXAMPLE 71 5-(4-Chlorobenzoyl)pyrrole-3-carboxylic Acid

Ethyl 5-(4-chlorobenzoyl)pyrrole-3-carboxylate (0.7 g.) was combinedwith 20 ml. of methanol and 15 ml. of 1N sodium hydroxide and boiled inan open flask for 1 hour. Most of the methanol evaporated. The mixturewas diluted to 40 ml. with water, treated with activated charcoal, andacidified to yield 5-(4-chlorobenzoyl)pyrrole-3-carboxylic acid [550mg., m.p. 278°-280° C. (dec)]. Recrystallization from 1:1methanol-acetone gave two crops of purified product [385 mg., m.p.280°-282° C. (dec)].

Analysis: Calcd. for C₁₂ H₈ O₃ NCl: C, 57.73; H, 3.23; N, 5.61. Found:C, 57.50; H, 3.15; N, 5.42.

By the same procedure, the other ethyl esters of Example 70 areconverted to:

5-Benzoylpyrrole-3-carboxylic acid;

5-(4-Fluorobenzoyl)pyrrole-3-carboxylic acid; and

5-(4-Methylbenzoyl)pyrrole-3-carboxylic acid.

EXAMPLE 72 Methyl 5-(2,4-Dichlorobenzoyl)pyrrole-3-carboxylate

By the procedure of Example 64, 2,4-dichlorobenzoyl chloride (4.18 g.,20 mmoles) and methyl pyrrole-3-carboxylate were reacted to form methyl5-(2,4-dichlorobenzoyl)pyrrole-3-carboxylate (3.0 g., gummy solid, Rf0.5 on silica gel thin layer chromatography with ethylacetate-1/hexane-1 as eluant).

EXAMPLE 73 5-(2,4-Dichlorobenzoyl)pyrrole-3-carboxylic Acid

Methyl 5-(2,4-dichlorobenzoyl)pyrrole-3-carboxylate (3.0 g.) wasrefluxed for 2.5 hours in 35 ml. of methanol and 35 ml. of 1N sodiumhydroxide. The methanol was evaporated, and approximately one volume ofwater was added to the aqueous residue. The mixture was extracted twicewith ether, and acidified with conc. hydrochloric acid to yield 2.12 g.of crude product, recovered by filtration. Recrystallization frommethanol, which included an activated carbon treatment, affordedpurified 5-(2,4-dichlorobenzoyl)pyrrole-3-carboxylic acid (1.06 g., m.p.245°-249° C.).

Analysis: Calcd. for C₁₂ H₇ O₃ NCl₂ : C, 50.73; H, 2.48; N, 4.93. Found:C, 50.66; H, 2.69; N, 4.87.

EXAMPLE 74 Methyl 5-(3,5-Dimethoxybenzoyl)pyrrole-3-carboxylate

By the procedure of Example 64, but a 5 hour reaction time,3,5-dimethoxybenzoyl chloride (4.0 g., 20 mmoles) was reacted withmethyl pyrrole-3-carboxylate to yield, without recrystallization, methyl5-(3,5-dimethoxybenzoyl)pyrrole-3-carboxylate (3.7 g., m.p. 115°-120°C., m/e 289).

EXAMPLE 75 5-(3,5-Dimethoxybenzoyl)pyrrole-3-carboxylic Acid

Methyl 5-(3,4-dimethoxybenzoyl)pyrrole-3-carboxylate (3.7 g.) wasrefluxed with 40 ml. of methanol and 40 ml. of 1N sodium hydroxide with2 hours and isolated by the procedure of Example 73. Recrystallizationfrom methanol gave purified 5-(3,5-dimethoxybenzoyl)pyrrole-3-carboxylicacid (1.5 g., m.p. 267°-269° C.).

Analysis: Calcd. for C₁₄ H₁₃ O₅ N: C, 61.08; H, 4.75; N, 5.08. Found: C,60.65; H, 4.72; N, 5.11.

EXAMPLE 76 2-(4-Phenylbenzoylmethyl)imidazo[3,4-a]pyridinium Bromide

Imidazo[3,4-a]pyridine (7.1 g., 60 mmoles), dissolved in 150 ml. ofether, was mixed with p-phenylphenacyl bromide dissolved in 100 ml. oftetrahydrofuran and stirred for 4 hours at room temperature. A firstcrop of crystalline 2-(4-phenylbenzoylmethyl)imidazo[3,4-a]pyridiniumbromide [7.8 g., m.p. 258°-260° C. (dec.)] was recovered by filtration.After standing four days at room temperature, a second crop ofcrystallized product [9.5 g., m.p. 262°-264° C. (dec.)] was recoveredfrom the filtrate.

EXAMPLE 77 Ethyl1-(2-Pyridylmethyl-5-(4-phenylbenzoyl)pyrrole-3-carboxylateHydrochloride

2-(4-Phenylbenzoylmethyl)imidazo[3,4-a]pyridinium bromide (16 g., 40mmoles), potassium carbonate (22 g., 160 mmoles) and ethyl propiolate(6.4 ml., 48 mmoles) was combined according to the procedure of Example69, stirred for approximately 16 hours at room temperature, thenisolated and converted to hydrochloride according to the procedure ofExample 69, to yield ethyl1-(2-pyridylmethyl)-5-(4-phenylbenzoyl)pyrrole-3-carboxylate (5.3 g.,intermediate Rf on silica gel thin layer chromatography with ethylacetate/5% acetic acid as eluant).

EXAMPLE 78 Ethyl 5-(4-Phenylbenzoyl)pyrrole-3-carboxylate

Ethyl 1-(2-pyridylmethyl)-5-(4-phenylbenzoyl)pyrrole-3-carboxylate (5.3g.) was combined with 150 ml. of dioxane, 5 ml. of water and 10 g. ofselenium dioxide and refluxed for approximately 16 hours. Insolublematerial was separated by filtration, the filtrate was diluted with 150ml. of ether and 150 ml. of water, the mixture equilibrated, the etherphase separated, back-washed twice with 100 ml. portions of water,filtered and concentrated to yield ethyl5-(4-phenylbenzoyl)pyrrole-3-carboxylate (3.1 g, solid; Rf 0.4 on silicagel thin layer chromatography with ethyl acetate-1/hexane-5/5% aceticacid as eluant).

By following the procedure of Examples 64, 66, 68 and 74,4-phenylbenzoyl chloride and 2-phenylbenzoyl chloride are reacted withmethyl pyrrole-3-carboxylate to yield similar compounds, viz.:

Methyl 5-(4-phenylbenzoyl)pyrrole-3-carboxylate; and

Methyl 5-(2-phenylbenzoyl)pyrrole-3-carboxylate.

EXAMPLE 79 5-(4-Phenylbenzoyl)pyrrole-3-carboxylic Acid

Ethyl 5-(4-phenylbenzoyl)pyrrole-3-carboxylate (3.1 g.) was boiled in anopen flask with 100 ml. of methanol and 70 ml. of 1N sodium hydroxidefor 1 hour by which time most of the methanol was removed byevaporation. The solution was cooled to room temperature, and productwhich had precipitated as the sodium salt recovered by filtration. Thesalt was stirred with 75 ml. of 1N hydrochloric acid and5-(4-phenylbenzoyl)pyrrole-3-carboxylic acid (1 g., m.p.>280° C.)recovered by filtration.

Analysis: Calcd. for C₁₈ H₁₃ O₃ N: C, 74.21; H, 4.50; N, 4.81. Found: C,73.86; H, 4.61; N, 4.91.

EXAMPLE 80 Methyl 5-Phenylacetylpyrrole-3-carboxylate

Under nitrogen, methyl pyrrole-3-carboxylate (920 mg., 7 mmoles) wasdissolved in 25 ml. of methylene chloride. Phenylacetyl chloride (930mg., 6 mmoles) was added and dissolved. Finally, stannic chloride (1.2ml., 12 mmoles) was added. The reaction mixture was stirred for 3 hours,water (15 ml.) added dropwise, diluted with 50 ml. of ether, the organicphase separated, washed with 20 ml. of water and evaporated to drynessto yield methyl 5-phenylacetylpyrrole-3-carboxylate (semisolid, Rf 0.3on silica gel thin layer chromatography with ethyl acetate-1/hexane-5/5%acetic acid as eluant). The entire batch was employed without weightdetermination in the next step.

EXAMPLE 81 5-Phenylacetylpyrrole-3-carboxylic Acid

All of the methyl 5-phenylacetylpyrrole-3-carboxylate prepared byExample 80 was boiled with methanol (20 ml.) and 1N sodium hydroxide (15ml.) for 90 minutes. The aqueous residue was cooled, acidified withconc. hydrochloric acid, and crude product (1.2 g.) recovered byfiltration. Chromatography on 30 g. of silica gel, employing ethylacetate-1/hexane-5/5% acetic acid as eluant, gave, on evaporation of theinitial, ultraviolet absorbing fractions, purified5-phenylacetylpyrrole-3-carboxylic acid (470 mg., m.p. 205°-207° C., m/e229).

Analysis: Calcd. for C₁₃ H₁₁ O₃ N.0.25H₂ O: C, 67.37; H, 5.00; N, 6.04.Found: C, 66.97; H, 4.99; N, 5.98.

EXAMPLE 82 Methyl 5-Cyclohexylcarbonylpyrrole-3-carboxylate

To a solution of methyl pyrrole-3-carboxylate (1.25 g., 10 mmoles) in 25ml. of methylene chloride under nitrogen, was added cyclohexanecarbonylchloride (1.46 g., 1.33 ml., 10 mmoles) and then stannic chloride (3.13g., 1.4 ml., 14 mmoles). The resulting solution was stirred 3 hours atroom temperature, then 15 ml. of water added slowly and finally 50 ml.of ether. The organic phase was separated, washed with 20 ml. of water,filtered and evaporated to yield methyl5-cyclohexylcarbonylpyrrole-3-carboxylate (Rf 0.25 on silica gel thinlayer chromatography with ethyl acetate-1/hexane-5/5% acetic acid aseluant). The entire batch was used, without weight determination, in thenext step.

By the same procedure, cyclopentylcarbonyl chloride andcycloheptylcarbonyl chloride are converted respectively to:

Methyl 5-Cyclopentylcarbonylpyrrole-3-carboxylate; and

Methyl 5-Cycloheptylcarbonylpyrrole-3-carboxylate.

EXAMPLE 83 5-Cyclohexylcarbonylpyrrole-3-carboxylic Acid

The entire batch of methyl 5-cyclohexylcarbonyl-3-carboxylate fromExample 82 was hydrolyzed and isolated according to the procedure ofExample 81 to yield crude product (1.1 g.). Recrystallization fromacetone afforded purified 5-cyclohexylcarbonylpyrrole-3-carboxylic acid(360 mg., m.p. 264°-265° C., m/e 221).

Analysis: Calcd. for C₁₂ H₁₅ O₃ N: C, 65.14; H, 6.83; N, 6.33. Found: C,65.28; H, 6.90; N, 6.46.

By the same procedure the other methyl esters of Example 82 areconverted to:

5-Cyclopentylcarbonylpyrrole-3-carboxylic acid; and

5-Cycloheptylcarbonylpyrrole-3-carboxylic acid.

EXAMPLE 84 Methyl 1-Methyl-5-benzoylpyrrole-3-carboxylate

Methyl 5-benzoyl-3-carboxylate (4.1 g., 18 mmoles) was dissolved withstirring in 500 ml. of ether. Sodium hydride (57%, 1.5 g., 36 mmoles)was added and the mixture stirred under nitrogen for 1.5 hours.Dimethylsulfate (3.5 ml., 40 mmoles) was then added and stirringcontinued for approximately 16 hours. Water (100 ml.) was added slowly,still under nitrogen, and the mixture stirred for 15 minutes. The etherlayer was separated, washed with 100 ml. of water and concentrated todryness to yield methyl 1-methyl-5-benzoyl-3-carboxylate (Rf 0.45 onsilica gel thin layer chromatography with ethyl acetate-1/hexane-5/5%acetic acid as eluant). The entire batch of product was used, withoutweight determination, in the next step.

By the same procedure, other 5-acylpyrrole-3-carboxylic esters preparedin Examples above are converted to:

Methyl 1-methyl-5-(2-methylbenzoyl)pyrrole-3-carboxylate;

Methyl 1-methyl-5-(4-methylbenzoyl)pyrrole-3-carboxylate;

Ethyl 1-methyl-5-(4-chlorobenzoyl)pyrrole-3-carboxylate;

Methyl 1-methyl-5-(2,3-dichlorobenzoyl)pyrrole-3-carboxylate;

Ethyl 1-methyl-5-(4-phenylbenzoyl)pyrrole-3-carboxylate;

Methyl 1-methyl-5-phenylacetylpyrrole-3-carboxylate; and

Methyl 1-methyl-5-cyclohexylcarbonylpyrrole-3-carboxylate.

Belgian Pat. No. 870,910 (published Mar. 29, 1979) discloses preparationof analogous 1-alkyl-5-acylpyrrole-3-carboxylates, specificallyexemplifying preparation of methyl1-methyl-5-(3-chlorobenzoyl)pyrrole-3-carboxylate by Friedel-Craftsacylation of 1-methylpyrrole-3-carboxylic acid followed byesterification and separation from the 4-acyl isomer.

EXAMPLE 85 1-Methyl-5-benzoylpyrrole-3-carboxylic Acid

The entire batch of methyl 1-methyl-5-benzoylpyrrole-3-carboxylate fromExample 84 was combined with 200 ml. of ethanol and 100 ml. of 1N sodiumhydroxide and refluxed for 2 hours. The ethanol was boiled away, theaqueous residue was cooled to room temperature, extracted with 25 ml. ofether and acidified with conc. hydrochloric acid to yield crystallineproduct (4 g., m.p. 210°-212° C.). A portion (1.5 g.) was recrystallizedfrom acetone to yield purified 1-methyl-5-benzoylpyrrole-3-carboxylicacid (925 mg., m.p. 215°-216.5° C., m/e 229).

By the same procedure, the other esters of Example 84 are converted to:

1-Methyl-5-(2-Methylbenzoyl)-pyrrole-3-carboxylic acid;

1-Methyl-5-(4-Methylbenzoyl)pyrrole-3-carboxylic acid;

1-Methyl-5-(4-Chlorobenzoyl)pyrrole-3-carboxylic acid;

1-Methyl-5-(2,3-Dichlorobenzoyl)pyrrole-3-carboxylic acid;

1-Methyl-5-(4-Phenylbenzoyl)pyrrole-3-carboxylic acid;

1-Methyl-5-Phenylacetylpyrrole-3-carboxylic acid; and

1-Methyl-5-Cyclohexylcarbonyl-3-carboxylic acid.

Belgian Pat. No. 870,910 (cited above) discloses preparation ofanalogous 1-alkyl-5-acylpyrrole-3-carboxylic acids, specificallypreparation of 1-methyl-5-(3-chlorobenzoyl)pyrrole-3-carboxylic acid bythe potassium hydroxide catalyzed hydrolysis of the corresponding methylester.

EXAMPLE 86 Ethyl 1-Ethyl-5-benzoylpyrrole-3-carboxylate

Ethyl 5-benzoylpyrrole-3-carboxylate (0.973 g., 4 mmoles) was dissolvedin 40 ml. of dry tetrahydrofuran. To the stirred solution, undernitrogen, there was added sodium hydride dispersion in oil (57%, 0.173g., 4 mmoles), and the mixture was stirred for 0.5 hour. Ethyl iodide(1.25 g., 8 mmoles) was added and stirring continued for 6 hours.Additional sodium hydride (0.17 g.) and ethyl iodide (1.25 g.) was addedand reflux continued for an additional 104 hours. Like quantities ofsodium hydride and ethyl iodide were added after 16 hours reflux and alike quantity of ethyl iodide after 24 hours reflux. The reaction wascooled and excess sodium hydride decomposed by cautious addition of 40ml. of water. The product was extracted into 40 ml. of ether, the etherback-washed with 10 ml. of water, and evaporated to dryness to yieldethyl 1-ethyl-5-benzoylpyrrole-3-carboxylate (1 g.), used directly inthe next step.

By the same procedure, other 5-acylpyrrole-3-carboxylic esters preparedin Examples above are converted to:

Methyl 1-ethyl-5-(2-methylbenzoyl)pyrrole-3-carboxylate;

Methyl 1-ethyl-(4-methylbenzoyl)pyrrole-3-carboxylate;

Ethyl 1-ethyl-5-(4-chlorobenzoyl)pyrrole-3-carboxylate;

Methyl 1-ethyl-5-(2,3-dichlorobenzoyl)pyrrole-3-carboxylate;

Methyl 1-ethyl-5-(4-phenylbenzoyl)pyrrole-3-carboxylate;

Methyl 1-ethyl-5-phenylacetylpyrrole-3-carboxylate; and

Methyl 1-ethyl-5-cyclohexylcarbonylpyrrole-3-carboxylate.

EXAMPLE 87 1-Ethyl-5-benzoylpyrrole-3-carboxylic Acid

The entire batch of ethyl 1-ethyl-5-benzoylpyrrole-3-carboxylate fromthe preceding Example was taken up in 40 ml. of methanol and 20 ml. of1N sodium hydroxide, and heated in an open flask on a steam bath for 1hour. The aqueous residue was cooled, extracted with 20 ml. of ether,and made acidic with conc. hydrochloric acid.1-Ethyl-5-benzoylpyrrole-3-carboxylic acid (585 mg., m.p. 157°-158° C.,m/e 243) was recovered by filtration. For analysis, the product wasrecrystallized from acetone/hexane (380 mg., m.p. 158°-160° C.).

Analysis: Calcd. for C₁₄ H₁₃ O₃ N: C, 69.12; H, 5.39; N, 5.76. Found: C,68.90; H, 5.09; N, 5.64.

EXAMPLE 88 Ethyl 4-Methylpyrrole-3-carboxylate

Ethyl 4-methylpyrrole-3-carboxylate was prepared according to theprocedure of Cheng et al. [J. Heterocyclic Chem. 13, 1145 (1976)].

EXAMPLE 89 Ethyl 4-Methyl-5-benzoylpyrrole-3-carboxylate

Ethyl 4-methylpyrrole-3-carboxylate (920 mg., 6 mmoles) was combinedwith benzoyl chloride (0.7 ml., 6 mmoles), stannic chloride (1.2 ml., 12mmoles) in 25 ml. of methylene chloride and stirred under nitrogen for 2hours at room temperature. Water (15 ml.) was added slowly, dissolvingthe precipitate which had formed. Ether (50 ml.) was then added, and theorganic phase separated, back-washed with 20 ml. of water, andevaporated to an oil. The oil was triturated with hexane to yieldcrystalline product (1.12 g.). Recrystallization from acetone/hexaneafforded purified ethyl 4-methyl-5-benzoylpyrrole-3-carboxylate (900mg., m.p. 127°-129° C.).

EXAMPLE 90 4-Methyl-5-benzoylpyrrole-3-carboxylic Acid

Ethyl 4-methyl-5-benzoylpyrrole-3-carboxylate (0.8 g.) was combined with20 ml. of methanol and 15 ml. of 1N sodium hydroxide and boiled in anopen flask for 1.5 hours on a steam bath. The aqueous residue wascooled, extracted with 10 ml. of ether, and acidified with conc.hydrochloric acid to yield crystalline4-methyl-5-benzoylpyrrole-3-carboxylic acid (630 mg., m.p. 241°-243° C.,m/e 229).

Analysis: Calcd. for C₁₃ H₁₁ O₃ N.0.125H₂ O: C, 67.45, H, 4.89; N, 6.05.Found: C, 67.18; H, 5.14; N, 5.83.

EXAMPLE 91 Methyl 4-Phenylpyrrole-3-carboxylate

p-Tosylmethyl isocyanide (1 g., 5 mmoles) and methyl cinnamate (0.8 g.,5 mmoles) were combined in 50 ml. of tetrahydrofuran and dissolved bystirring at room temperature. The solution was cooled to -50° C. andsodium hydride (57% dispersion in oil, 210 mg., 5 mmoles) added. Afterstirring for 15 minutes at -50° C., the reaction was warmed to roomtemperature and stirred for approximately 16 hour. Water (20 ml.) wasadded and the product extracted into 35 ml. ether. The ether wasback-washed with 20 ml. of water, evaporated to dryness, the residuetriturated with ether and methyl 4-phenylpyrrole-3-carboxylate (230 mg.,m.p. 182°-185° C., m/e 201) recovered by filtration. The product wasrecrystallized from acetone/hexane (210 mg., m.p. 184°-187° C.).

Analysis: Calcd. for C₁₂ H₁₁ O₂ N: C, 71.62; H, 5.51; N, 6.96. Found: C,71.37; H, 5.55; N, 7.12.

EXAMPLE 92 Methyl 4-Phenyl-5-benzoylpyrrole-3-carboxylate

Methyl 4-phenylpyrrole-3-carboxylate (920 mg., 4.6 mmoles) and benzoylchloride (0.53 ml., 4.6 mmoles) were combined in 50 ml. methylenechloride and stirred under nitrogen at room temperature. Stannicchloride (1.15 ml., 10 mmoles) was added. The resulting solution wasstirred for 1.5 hours. Hydrochloric acid (1N, 25 ml.) was added slowly.The mixture was stirred for 10 minutes, the methylene chloride layerseparated, back-washed with two 20 ml. portions of water, approximately50 mg. of solids removed by filtration, and the filtrate evaporated todryness. Recrystallization of the residue from acetone afforded purifiedmethyl 4-phenyl-5-benzoylpyrrole-3-carboxylate (673 mg., m.p. 201°-203°C.).

Analysis: Calcd. for C₁₉ H₁₅ O₃ N: C, 74.74; H, 4.95; N, 4.59. Found: C,74.67; H, 4.85; N, 4.51.

EXAMPLE 93 4-Phenyl-5-benzoylpyrrole-3-carboxylic Acid

Methyl 4-phenyl-5-benzoylpyrrole-3-carboxylate (345 mg.) was combinedwith 20 ml. of methanol and 10 ml. of 1N sodium hydroxide and boiled inan open flask for 1 hour. The aqueous residue was cooled, extracted withethyl acetate, acidified with conc. hydrochloric acid, and productrecovered by filtration. Recrystallization from tetrahydrofuran/hexaneafforded purified 4-phenyl-5-benzoylpyrrole-3-carboxylic acid [63 mg.,m.p. 285°-287° C. (dec.)].

Analysis: Calcd. for C₁₈ H₁₃ O₃ N.0.33H₂ O: C, 72.71; H, 4.63; N, 4.70.Found: C, 72.62; H, 4.52; N, 4.76.

EXAMPLE 94 5-Benzylpyrrole-3-carboxylic Acid

5-Benzoyl-3-carboxylic acid (0.6 g.) was combined with 4 ml. ofdiethyleneglycol, 2 g. of potassium hydroxide and 1.5 ml. of 97%hydrazine and heated in an oil bath for 1 hour at 135° C. and then 1hour at 173°-175° C. The reaction mixture was cooled to roomtemperature, diluted with 30 ml. of water, acidified with conc.hydrochloric acid, and product (350 mg.) recovered by filtration.Recrystallization from ether/hexane afforded purified5-benzylpyrrole-3-carboxylic acid [160 mg., m.p. 123°-125° C. (turbid),m/e 201].

Analysis: Calcd. for C₁₂ H₁₁ O₂ N: C, 71.62; H, 5.51; N, 6.96. Found: C,71.32; H, 5.55; N, 7.14.

EXAMPLE 95 Methyl 4-Benzoylpyrrole-3-carboxylate

Methyl 3-benzoylacrylate (6.5 g., 33 mmoles) and tosylmethyl isocyanide(6.7 g., 33 mmoles) were dissolved in a mixture of 100 ml. of ether and40 ml. of dimethylsulfoxide and added dropwise to a suspension of sodiumhydride (57% suspension in oil, 2.8 g., 66 mmoles) stirring in 50 ml. ofether, under nitrogen. Addition required 20 minutes, a slightlyexothermic reaction being noted. The reaction was stirred for a further30 minutes at room temperature and diluted with 400 ml. of water. Theproduct was extracted into 150 ml. of ether, the ether back-washed with250 ml. of water, concentrated to approximately 50 ml., and methyl4-benzoylpyrrole-3-carboxylate (2.0 g, m.p. 164°-166° C.) recovered byfiltration.

Analysis: Calcd. for C₁₃ H₁₁ O₃ N: C, 68.11; H, 4.84; N, 6.11. Found: C,68.00; H, 4.69; N, 6.00.

By the same procedure, methyl 3-(4-methoxybenzoyl)acrylate, methyl3-(4-methylbenzoyl)acrylate, methyl 3-(3-chlorophenylbenzoyl)acrylateand methyl 3-cyclohexylcarbonyl)acrylate are converted, respectively,to:

Methyl 4-(4-methoxybenzoyl)pyrrole-3-carboxylate;

Methyl 4-(4-methylbenzoyl)pyrrole-3-carboxylate;

Methyl 4-(4-phenylbenzoyl)pyrrole-3-carboxylate;

Methyl 4-(3-chlorobenzoyl)pyrrole-3-carboxylate; and

Methyl 4-cyclohexylcarbonylpyrrole-3-carboxylate.

Belgian Pat. No. 870,910 (published Mar. 29, 1979) generally disclosesanalogous compounds, specifically examplifying the preparation of methyl1-methyl-4-(3-chlorobenzoyl)pyrrole-3-carboxylate by Friedel-Craftsacylation of 1-methylpyrrole-3-carboxylate.

EXAMPLE 96 4-Benzoylpyrrole-3-carboxylic Acid

Methyl 4-benzoylpyrrole-3-carboxylate (1 g.) was combined with 30 ml. ofmethanol and 20 ml. of 1N sodium hydroxide and boiled in an open flaskfor 1 hour. The aqueous residue was diluted with 20 ml. of water,acidified with concentrated hydrochloric acid, and crystalline4-benzoylpyrrole-3-carboxylic acid (800 mg., m.p. 220°-222° C.)recovered by filtration.

Analysis: Calcd. for C₁₂ H₉ O₃ N.0.5H₂ O: C, 64.28; H, 4.49; N, 6.25.Found: C, 64.10; H, 4.05; N, 6.08.

Anhydrous material was obtained by recrystallization from acetone/hexane(m.p. 222°-224° C.).

Analysis: Calcd. for C₁₂ H₉ O₃ N: C, 66.97; H, 4.22; N, 6.51. Found: C,66.86; H, 4.14; N, 6.38.

By the same procedure, the other pyrrole esters of Example 95 areconverted to:

4-(4-Methoxybenzoyl)pyrrole-3-carboxylic acid;

4-(4-Methylbenzoyl)pyrrole-3-carboxylic acid;

4-(4-Phenylbenzoyl)pyrrole-3-carboxylic acid;

4-(3-Chlorobenzoyl)pyrrole-3-carboxylic acid; and

4-Cyclohexylcarbonylpyrrole-3-carboxylic acid.

Belgian Pat. No. 870,910 (cited above) generally discloses analogouscompounds, specifically exemplifying the preparation of1-methyl-4-(3-chlorobenzoyl)pyrrole-3-carboxylic acid by potassiumhydroxide catalyzed hydrolysis of the corresponding ester.

EXAMPLE 97 Methyl 5-Chloropyrrole-3-carboxylate

Methyl pyrrole-3-carboxylate (2.5 g., 20 mmoles) was dissolved in 50 ml.of methylene chloride. The solution, stirred under nitrogen, was cooledin an ice-water bath while t-butylhypochlorite (1.95 g., 18 mmoles) in25 ml. of methylene chloride was added dropwise. The reaction wasstirred for 1 hour at room temperature, filtered and evaporated to anoil. The oil was chromatographed (100 g. of silica gel with ethylacetate-1/hexane-7 as eluant). The column elution was monitored by thinlayer chromatography (silica gel plates with chloroform-8/ethylacetate-2 as eluant). Cuts containing pure product (Rf 0.4) werecombined and evaporated to yield crystalline methyl5-chloropyrrole-3-carboxylate (900 mg., m.p. 92°-95° C., m/e 159/161).

EXAMPLE 98 5-Chloropyrrole-3-carboxylic Acid

Methyl 5-chloropyrrole-3-carboxylate (900 g.) was refluxed for 9 hourswith 20 ml. of methanol and 10 ml. of 1N sodium hydroxide. Methanol wasremoved by evaporation, the aqueous residue was diluted withapproximately 10 ml. of water and extracted twice with ether. Theaqueous phase was acidified with conc. hydrochloric acid and productextracted into ethyl acetate. The three ethyl acetate extracts werecombined, washed with saturated sodium chloride, dried over anhydroussodium sulfate and evaporated to product (715 mg., m.p. 175°-178° C.).Recrystallization from methylene chloride/hexane afforded purified5-chloropyrrole-3-carboxylic acid (300 mg., m.p. 178°-180° C.).

Analysis: Calcd. for C₅ H₄ O₂ NC: C, 41.26; H, 2.76; N, 9.62. Found: C,41.43; H, 2.87; N, 9.57.

EXAMPLE 99 Methyl 1-Methyl-5-chloropyrrole-3-carboxylate

Methyl 5-chloropyrrole-3-carboxylate (800 mg., 5 mmoles) was dissolvedin 75 ml. of ether. Sodium hydride (57% dispersion in oil, 430 mg., 10mmoles) was added and the mixture stirred under nitrogen for 1 hour.Dimethyl sulfate (1 ml., 10.7 mmoles) was added and stirring continuedfor 2 hours. Excess sodium hydride was decomposed by cautious additionof 20 ml. of water. The ether phase was washed with 20 ml. of water andevaporated to yield methyl 1-methyl-5-chloropyrrole-3-carboxylate (oil);the entire batch being used directly in the next step.

EXAMPLE 100 1-Methyl-5-chloropyrrole-3-carboxylic Acid

Methyl 1-methyl-5-chloropyrrole-3-carboxylate from the previous examplewas hydrolyzed in approximately 20 ml. of 1N sodium hydroxide and 30 ml.of ethanol, by boiling on a steam bath for approximately 2 hours. Theaqueous residue was diluted with approximately 20 ml. of water,acidified with conc. hydrochloric acid, and crystalline1-methyl-5-chloropyrrole-3-carboxylic acid recovered by filtration (590mg., m.p. 201°-203° C.).

Analysis: Calcd. for C₆ H₆ O₂ NCl: C, 45.16; H, 3.79; N, 8.78. Found: C,45.01; H, 3.81; N, 8.66.

EXAMPLE 101 4-Benzylpyrrole-2-carboxylic Acid

4-Benzoylpyrrole-2-carboxylic acid (1.5 g.) was combined with 10 ml. ofethylene glycol, 4 ml. of hydrazine (97%) and 4 g. of potassiumhydroxide, and heated for 2 hours in an oil bath maintained at 140°-143°C. The reaction mixture was poured into approximately 100 ml. of ice andwater, acidified with conc. hydrochloric acid, and product (375 mg.)recovered by filtration. Recrystallization from ether/hexane affordedpurified 4-benzoylpyrrole-2-carboxylic acid (200 mg., m.p. 183°-185°C.).

Analysis: Calcd. for C₁₂ H₁₁ O₂ N: C, 71.62; H, 5.51; N, 6.96. C, 71.52;H, 5.71; N, 7.13.

EXAMPLE 102 2-Hydroxyethyl 4-(4-Chlorophenoxy)pyrrole-2-carboxylate

2-(4-Chlorophenoxy)-3-carbethoxymethylaminoacrylaldehyde (2 g.) wasboiled in 5 ml. of ethylene glycol for 15 minutes. The reaction wascooled to room temperature, diluted with 10 ml. of water and productextracted into 10 ml. of ethyl acetate. The ethyl acetate wasback-washed with water and concentrated to an oil (1.5 g.). The oilchromatographed on approximately 100 ml. of silica gel with ethylacetate-1/hexane-1 as eluant, monitored by thin layer chromatography.Evaporation of middle fractions gave ethyl4-(4-chlorophenoxy)pyrrole-2-carboxylate (100 mg., oil, m/e 281, Rf 0.65on silica gel thin layer chromatography with ethyl acetate-1/hexane-1/5%acetic acid as eluant; Rf 0.3 with ethyl acetate-1/hexane-1 as eluant).Repeat on a 5.5 g. scale gave an additional 400 mg. of product.

EXAMPLE 103 4-(4-Chlorophenoxy)pyrrole-2-carboxylic Acid

2-Hydroxyethyl 4-(4-chlorophenoxy)pyrrole-2-carboxylate (400 mg.) wasdissolved in 10 ml. of acetone and 10 ml. of 1N sodium hydroxide andleft to stand at room temperature for approximately 16 hours. Acetonewas evaporated in vacuo, the aqueous residue was acidified with conc.hydrochloric acid and crystalline product (320 mg.) recovered byfiltration. Recrystallization from ether/hexane of 400 mg. of productprepared in this manner afforded purified4-(4-chlorophenoxy)pyrrole-2-carboxylic acid (234 mg., m.p. 188°-190°C., m/e 237).

Analysis: Calcd. for C₁₁ H₈ O₃ NCl: C, 55.59; H, 3.39; N, 5.89. Found:C, 55.92; H, 3.48; N, 5.82.

EXAMPLE 104 2-Hydroxyethyl 4-(4-Chlorophenyl)pyrrole-2-carboxylate

2-(4-Chlorophenyl)-3-carbethoxymethylaminoacrylaldehyde (73.6 g.) wasrefluxed in 300 ml. of ethylene glycol for 10 minutes. The reactionmixture was cooled, diluted with 1 liter of water, product extractedinto chloroform, treated with activated carbon and evaporated to solids.Trituration with ether gave crude product (8 g.). An additional quantityof crude product (7.5 g.) crystallized from the aqueous phase which hadbeen standing. Recrystallization of the combined crude solids fromchloroform gave 2-hydroxyethyl 4-(4-chlorophenyl)pyrrole-2-carboxylate(5 g., m.p. 128°-130° C.). The ether and chloroform mother liquors werecombined and evaporated to yield an additional 40 g. of crude product.

EXAMPLE 105 4-(4-Chlorophenyl)pyrrole-2-carboxylic Acid

Crude 2-hydroxyethyl 4-(4-chlorophenyl)pyrrole-2-carboxylate (3 g.) washeated on a steam bath with potassium hydroxide (2 g.) in 25 ml. ofwater and 25 ml. of ethanol for 30 minutes. The balance of the ethanolwas evaporated in vacuo, chloroform (25 ml.) was added and4-(4-chlorophenyl)pyrrole-2-carboxylic acid (723 mg., m.p. 219°-222° C.,ir(KBr): 3390, 3077 and 1695 cm.⁻¹, m/e 221/223) recovered byfiltration.

EXAMPLE 106 4-Phenylpyrrole-2-carboxylic Acid

4-(4-Chlorophenyl)pyrrole-2-carboxylic acid (0.6 g.) was hydrogenatedover 200 mg. of 5% Pd/C in 50 ml. of ethanol for approximately 16 hoursat 50 p.s.i. An additional 200 ml. of catalyst and 1 ml. oftriethylamine was added and hydrogenation continued for 3 hours at 50p.s.i. The catalyst was recovered by filtration, and the mother liquorconcentrated to dryness to yield 4-phenylpyrrole-2-carboxylic acid [400mg., m.p. 226°-228° C. (dec.), m/e 187]. For analysis, the product wasrecrystallized from acetone/hexane [240 mg., m.p. 227°-229° C. (dec.)].

Analysis: Calcd. for C₁₁ H₉ O₂ N.0.125H₂ O: C, 69.73; H, 4.92; N, 7.39.Found: C, 70.06; H, 4.99; N, 7.26.

EXAMPLE 107 Methyl Pyrrole-2-Carboxylate

Pyrrole-2-carboxylic acid (5 g.) was combined with 200 ml. of methylenechloride and 20 ml. of thionyl chloride and refluxed for 2 hours. Thesolution was evaporated to dryness in vacuo and the resulting acidchloride converted to ester by addition of 30 ml. of methanol and 20minutes stirring at room temperature. Ether (50 ml.) was added and themixture extracted with 50 ml. of water. The ether phase was dried overanhydrous sodium sulfate, filtered and evaporated to yield methylpyrrole-2-carboxylate (5 g., m.p. 69°-71° C.).

EXAMPLE 108 Methyl 5-Benzoylpyrrole-2-carboxylate

Methylpyrrole-2-carboxylate (1.2 g., 10 mmoles) and benzoyl chloride(1.2 ml., 10 mmoles) were dissolved in 25 ml. of methylene chloride andadded, over 2 minutes, to a solution of stannic chloride (2.6 ml., 22mmoles) in 25 ml. of methylene chloride. After stirring for 2 hours atroom temperature, 1N hydrochloric acid (25 ml.) was added slowly and themixture was stirred for 15 minutes. The methylene chloride phase wasseparated, back-washed twice with 25 ml. portions of water, andevaporated to yield crude methyl 5-benzoylpyrrole-2-carboxylate (2.3g.). Chromatography of approximately 3 g. of product prepared in thismanner on silica gel with ethyl acetate-1/hexane-2 as eluant, followedby recrystallization of the 2.6 g. thereby obtained from isopropylalcohol gave 0.75 g. of purified product in two crops (m.p. 111°-113°C.).

Analysis: Calcd. for C₁₃ H₁₁ O₃ N: C, 68.11; H, 4.84; N, 6.11. Found: C,67.80; H, 4.81; N, 6.11.

Alternatively, purified product (5.9 g.) was obtained by fractionalcrystallization of 18.3 g. of crude from isopropyl alcohol. Alsoobtained was methyl 4-benzoylpyrrole-2-carboxylic acid (5.2 g., m.p.135°-137° C.), a compound [Sanchez et al., Carbohydrate Res. 3, 486(1967)] previously derived from an amino sugar.

By the same procedure, appropriate acid chlorides are reacted withmethyl pyrrole-2-carboxylate to yield:

Methyl 5-(2-methylbenzoyl)pyrrole-2-carboxylate;

Methyl 5-(3-methylbenzoyl)pyrrole-2-carboxylate;

Methyl 5-(3-chlorobenzoyl)pyrrole-2-carboxylate;

Methyl 5-(2,5-dichlorobenzoyl)pyrrole-2-carboxylate;

Methyl 5-(3,5-dimethoxybenzoyl)pyrrole-2-carboxylate;

Methyl 5-(4-phenylbenzoyl)pyrrole-2-carboxylate;

Methyl 5-phenylacetylpyrrole-2-carboxylate; and

Methyl 5-cyclohexylcarbonylpyrrole-2-carboxylate.

The preparation of such acids is disclosed broadly in Belgian Pat. No.870,910, published Mar. 3, 1979. However, no specific compound of thisclass is described therein.

EXAMPLE 109 5-Benzoylpyrrole-2-carboxylic Acid

Methyl 5-benzoylpyrrole-2-carboxylate (460 mg.) was combined with 10 ml.of methanol and 20 ml. of 1N sodium hydroxide and heated in an openflask on a steam bath for 1 hour. On cooling the aqueous residue, sodium5-benzoylpyrrole-2-carboxylate (358 mg., m.p. >260° C., thin layerchromatography Rf 0.3 on silica gel with ethyl acetate-1/hexane-5/5%acetic acid as eluant) crystallized and was recovered by filtration. Thefiltrate was acidified with hydrochloric acid to yield additionalproduct as the free acid [96 mg., m.p. 227°-229° C. (dec.)]. Sodium saltwas converted to free acid by stirring in a mixture of 10 ml. ofmethanol and 20 ml. of 1N hydrochloric acid for 30 minutes [250 mg.,m.p. 229°-231° C. (dec)].

Analysis: Calcd. for C₁₂ H₉ O₃ N: C, 66.97; H, 4.22; N, 6.51. Found: C,66.89; H, 4.28; N, 6.49.

By the same procedure, the other esters of Example 108 are converted to:

5-(2-Methylbenzoyl)pyrrole-2-carboxylic acid;

5-(4-Methylbenzoyl)pyrrole-2-carboxylic acid;

5-(4-Chlorobenzoyl)pyrrole-2-carboxylic acid;

5-(2,5-Dichlorobenzoyl)pyrrole-2-carboxylic acid;

5-(3,5-Dimethoxybenzoyl)pyrrole-2-carboxylic acid;

5-(4-Phenylbenzoyl)pyrrole-2-carboxylic acid;

5-Phenylacetylpyrrole-2-carboxylic acid; and

5-Cyclohexylcarbonylpyrrole-2-carboxylic acid.

EXAMPLE 110 Methyl 1-Methyl-5-benzoylpyrrole-2-carboxylate

Under a nitrogen atmosphere, methyl 5-benzoylpyrrole-2-carboxylate (0.85g., 3.7 mmoles) was dissolved in 175 ml. of ether. To the stirredsolution there was added sodium hydride (57% dispersion in oil, 0.31 g.,7.4 mmoles), resulting in a yellow suspension. After stirring for 1 hourat room temperature, dimethylsulfate (0.95 g., 0.7 ml., 7.5 mmoles) wasadded and the reaction stirred for approximately 16 hours at roomtemperature. Identically-sized portions of sodium hydride anddimethylsulfate were added, and stirring continued for 8 hours; halfquantities of the same reagents were added and stirring continued for anadditional 16 hours. The reaction mixture was diluted dropwise withwater (20 ml.). The aqueous phase was separated and the organic phasewashed with an additional 20 ml. of water. The organic phase wasevaporated to dryness to yield solid product contaminated with the oilfrom the sodium hydride. Recrystallization from methanol gave purifiedmethyl 1-methyl-5-benzoylpyrrole-2-carboxylate (0.78 g., m.p. 111°- 113°C., m/e 243).

By the same procedure, the other esters of Example 108 are converted to:

Methyl 1-methyl-5-(2-methylbenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-(4-methylbenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-(4-chlorobenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-(3,5-dichlorobenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-(3,5-dimethoxybenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-(4-phenylbenzoyl)pyrrole-2-carboxylate;

Methyl 1-methyl-5-phenylacetylpyrrole-2-carboxylate; and

Methyl 1-methyl-5-cyclohexylcarbonylpyrrole-2-carboxylate.

EXAMPLE 111 1-Methyl-5-benzoylpyrrole-2-carboxylic Acid

Methyl 1-methyl-5-benzoylpyrrole-2-carboxylate (0.78 g.) was heated on asteam bath for 1 hour with 40 ml. of 1N sodium hydroxide and 20 ml. ofmethanol. The reaction mixture was cooled to room temperature, extractedwith ether, made acid with conc. hydrochloric acid and1-methyl-5-benzoylpyrrole-2-carboxylic acid recovered by filtration(0.47 g., m.p. 178°-181° C.). Recrystallization from acetone/hexaneafforded product for analysis (0.34 g., m.p. 178°-180° C.).

Analysis: Calcd. for C₁₃ H₁₁ O₃ N: C, 68.11; H, 4.84; N, 6.11. Found: C,67.82; H, 4.79; N, 6.01.

By the same process, the other esters of Example 110 are hydrolyzed toyield:

1-Methyl-5-(2-methylbenzoyl)pyrrole-2-carboxylic acid;

1-Methyl-5-(4-methylbenzoyl)pyrrole-2-carboxylic acid;

1-Methyl-5-(4-chlorobenzoyl)pyrrole-2-carboxylic acid;

1-Methyl-5-(3,5-dichlorobenzoyl)pyrrole-2-carboxylic acid;

1-Methyl-5-(4-phenylbenzoyl)pyrrole-2-carboxylic acid;

1-Methyl-5-phenylacetylpyrrole-2-carboxylic acid; and

1-Methyl-5-cyclohexylcarbonylpyrrole-2-carboxylic acid.

EXAMPLE 112 1-Methyl-4,5-dibromopyrrole-2-carboxylic Acid

1-Methylpyrrole-2-carboxylic acid (5 g., 40 mmoles) was dissolved in 100ml. of chloroform. A solution of bromine (2.3 ml., 44 mmoles) in 15 ml.of chloroform was added dropwise over 15 minutes. The reaction mixturewas then stirred at room temperature for 1 hour. Additional bromine (2.3ml., 44 mmoles) was added in one portion. A precipitate formed. Morechloroform (50 ml.) was added and stirring continued for 20 minutes.1-Methyl-4,5-dibromopyrrole-2-carboxylic acid was recovered byfiltration [5 g., m.p. 173°-175° C. (dec.), m/e 283]. One gram ofproduct was recrystallized from 1 to 1 acetone-water [614 mg., m.p.180°-182° C. (dec.)].

EXAMPLE 113 Sodium 5-Phenylthiopyrrole-3-carboxylate

5-Phenylthiopyrrole-3-carboxylate is combined with an equivalent ofsodium ethoxide in ethyl acetate. Sodium5-phenylthiopyrrole-3-carboxylate is isolated by concentration todryness or by precipitation resulting from addition of a non-solvent(ether or hexane).

Substitution of an equivalent amount of diethanol amine for sodiumethoxide is employed to produce diethanolammonium5-phenylthio-3-carboxylate.

EXAMPLE 114 Potassium 5-Butylthiopyrrole-3-carboxylate

5-Butylthiopyrrole-3-carboxylate is dissolved in ethyl acetate. Anequivalent of ethanolic potassium hydroxide is added. Potassium5-butylthiopyrrole-3-carboxylate is isolated by concentration to drynessor by preciptation resulting from addition of a non-solvent (ether orheptane).

Substitution of an equivalent of N-methylglucamine (meglumine) for theethanolic potassium hydroxide is employed to produceN-methylglucammonium 5-butylthiopyrrole-3-carboxylate.

EXAMPLE 115 Sodium 1-Methyl-5-benzoylpyrrole-3-carboxylate

1-Methyl-5-benzoylpyrrole-3-carboxylate is dissolved by warming inacetone. An equivalent of sodium methoxide is added with stirring.Sodium 1-methyl-5-benzoylpyrrole-3-carboxylate is isolated byevaporation to dryness or by precipitation resulting from addition of anon-solvent (ether or pentane).

Substitution of a molar equivalent of piperazine for sodium methoxide isemployed to produce piperazinium1-methyl-5-benzoylpyrrole-3-carboxylate.

EXAMPLE 116 Magnesium 4-(4-Chlorophenoxy)pyrrole-2-carboxylate

4-(4-Chlorophenoxy)pyrrole-2-carboxylate and an equivalent quantity ofmagnesium oleate are each dissolved in ethanol and the solutions mixed.Magnesium 4-(4-chlorophenoxy)pyrrole-2-carboxylate is isolated byconcentration and/or addition of heptane.

Substitution of an equivalent of calcium palmitate for magnesium oleatein this process is employed to produce calcium4-(4-chlorophenoxy)pyrrole-2-carboxylate.

EXAMPLE 117 Salt Formation

Alternatively, the acid products of Examples 1 to 112 are converted tothe sodium, potassium, ammonium, calcium, magnesium, aluminum,triethylamine, tri-n-butylamine, piperidine, triethanolamine,diethylaminoethylamine, pyrrolidine and N,N-dibenzylethylenediaminesalts by reaction with an equivalent of the appropriate metal hydroxide,ammonium hydroxide or amine in water or ethanol followed by filtrationof the salt if it is insoluble or by evaporation of the solvent if thesalt is soluble therein.

EXAMPLE 118 Capsules

A blend is prepared containing the following ingredients in theproportion by weight indicated:

    ______________________________________                                        Calcium carbonate, U.S.P.                                                                         17.6                                                      Dicalcium phosphate 18.8                                                      Magnesium trisilicate, U.S.P.                                                                     5.2                                                       Lactose, U.S.P.     5.2                                                       Potato starch       5.2                                                       Magnesium stearate A                                                                              0.8                                                       Magnesium stearate B                                                                              0.35                                                      ______________________________________                                    

To this blend is added sufficient sodium5-phenylthiopyrrole-3-carboxylate to fill standard size capsules so asto contain 500 mg., 300 mg., 100 mg., 50 mg. or 25 mg. of5-phenylthiopyrrole-3-carboxylic acid. The portion of blend to activedrug is within the limits of 1-0.1 to 1-2, i.e., 27.8 mg. of sodium saltand 250 mg. of blend in a 25 mg. carpsule or 556 mg. of sodium salt and250 mg. of blend in a 500 mg. capsule as examplary of the extremes.

EXAMPLE 119 Tablets

A tablet base is prepared by blending the following ingredients in theproportion by weight indicated:

    ______________________________________                                        Sucrose, U.S.P.   80.3                                                        Tapioca Starch    13.2                                                        Magnesium Stearate                                                                               6.5                                                        ______________________________________                                    

Into this tablet base there is blended sufficient sodium1-methyl-5-benzoylpyrrole-3-carboxylate to form tablets containing 50mg., 100 mg or 250 mg. of 1-methyl-5-benzoylpyrrole-3-carboxylic acid.The portion of blend to active drug is within the limits of 1-0.167 to1-1, i.e., 54.8 mg. of sodium salt and 300 mg. of blend in a 50 mg.tablet or 274 mg. of sodium salt and 250 mg. of blend in a 250 mg.tablet.

EXAMPLE 120 Injectable Preparation

A solution for parenteral, especially intramuscular injection isprepared with the following composition:

    ______________________________________                                        Magnesium 4-(4-chlorophenoxy)-                                                                        6.35    g.*                                           pyrrole-3-carboxylate                                                         Magnesium chloride hexahydrate                                                                        12.36   g.                                            Monoethanolamine        8.85    g.                                            Propylene glycol        376.00  g.                                            Water, distilled        94.00   g.                                            ______________________________________                                         *Weight equivalent to 6.04 g. 4(4-chlorophenoxy)pyrrole-3-carboxylate.   

The resultant solution has a concentration of effective ingredient of 10mg./ml.

EXAMPLE 121 Injectable Preparation

One hundred grams of sterile 5-(4-phenylbenzoyl)pyrrole-3-carboxylicacid is blended with 250 g. of sterile sodium ascorbate. The blend isdry filled into vials such that each vial contains 55 mg. of the activeingredient. Immediately before use, 11 ml. of sterile water forinjection is added to give a 5 mg./ml. solution suitable for intravenousinjection.

PREPARATION 1 2,4-Dichlorothiophenol

Following the procedure of French Pat. No. 1,481,052 [Chem. Abstr. 69,18840h (1968)], 2,4-dichloroaniline (32.4 g., 0.2 mole) was added to amixture of 200 ml. of conc. hydrochloric acid and approximately 200 g.of ice. The mixture was stirred at room temperature for 30 minutes;complete dissolution did not occur. The mixture was cooled to -2° C. anda solution of 15.2 g. of sodium nitrite (0.22 mole) in 50 ml. of waterwas added over a 10 minute period, maintaining the temperature between-2° and 2° C. during the addition. The reaction was stirred at 0°-2° C.for 30 minutes. Almost complete dissolution had occurred. This coldsolution was added portionwise over 15 minutes to a solution ofpotassium ethylxanthate (35.2 g., 0.22 mole) in 100 ml. of water,maintained at 45°-50° C. Heating at 50°- 55° C. was continued for 30minutes after addition was complete. The reaction was cooled to roomtemperature and the intermediate extracted into 200 ml. of ether. Theether phase was back-washed with water (150 ml.), with 1N sodiumhydroxide and twice more with 150 ml. portions of water, andconcentrated to yield 2,5-dichlorophenyl ethylxanthate (33 g. as anoil).

Without further purification the xanthate intermediate was combined with200 ml. of absolute ethanol and heated to reflux. Potassium hydroxide(85% pure, 12 g., 0.18 mole) was added in small portions over 30minutes. Reflux was continued for 2 hours. After cooling to roomtemperature, the reaction mixture was filtered to remove insolublebyproducts. To the filtrate was added 500 ml. of water, 200 ml. of etherand 100 ml. of hexane. The organic phase was separated and extractedwith 500 ml. of 0.5N sodium hydroxide. The combined aqueous phases wereacidified with conc. hydrochloric acid and the product extracted into150 ml. ether. The ether extract was back-washed with water, dried overanhydrous sodium sulfate and evaporated in vacuo to yield2,4-dichlorothiophenol (6.7 g.) as an oil.

PREPARATION 2 2,5-Dichlorothiophenol

Following the procedure of Preparation 1, 3,5-dichloroaniline (32.4 g.,0.2 mole) was reacted with sodium nitrite and then potassiumethylxanthate to form intermediate 3,5-dichlorophenyl ethylxanthate(25.5 g.). The latter was hydrolyzed and 3,5-dichlorothiophenyl (11.4g.) isolated following the further procedures of Preparation 1.

PREPARATION 3 Dicyclohexyl Disulfide

By the method of Frank and Blegen [Org. Syntheses 28, 16 (1948)],cyclohexyl mercaptan is oxidized by the action of hydrogen peroxide todicyclohexyl disulfide.

PREPARATION 4 Acid Chlorides

Acid chlorides required for use in various Examples of this patent areprepared by reaction of the corresponding acid (10 g.) with anequivalent of thionyl chloride in refluxing methylene chloride (100 ml.)for 2 hours in the presence of a catalytic amount of dimethylformamide(0.1 ml.). The acid chlorides are isolated by evaporation to dryness invacuo, and are purified by distillation, if necessary. In this manner,the following acid chlorides are prepared:

Cyclopentanecarbonyl chloride;

Cyclohexanecarbonyl chloride;

Cycloheptanecarbonyl chloride;

2-Phenylbenzoyl chloride;

4-Phenylbenzoyl chloride.

PREPARATION 5 2-Formamidomethylpyridine

2-Aminomethylpyridine (60 g.) was refluxed with 180 ml. of formic acidfor 4 hours and left to stir for approximately 16 hours at roomtemperature. The reaction mixture was concentrated to an oil andpurified; 2-formamidomethylpyridine obtained by distillation (72.3 g.,b.p. 117°-141° C./0.5 mm.)

PREPARATION 6 Imidazo[3,4-a]pyridine

2-Formamidomethylpyridine (39 g., 0.29 mole) was dissolved in 150 ml. ofbenzene. Phosphorus oxychloride (100 ml.) was added dropwise at such arate that the reaction began to reflux. By external heating, reflux wasmaintained for 3.5 hours. After standing approximately 16 hours at roomtemperature, the reaction was concentrated to an oil. The oil was pouredonto approximately 300 g. of ice, the mixture made basic (approximatelypH 10) with 10N sodium hydroxide, and extracted with three 300 ml.portions of methylene chloride. The aqueous was made more basic andextracted with a further three 300 ml. portions of methylene chloride.All of the extracts were combined, back-washed with 75 ml. of water andconcentrated to yield crystalline imidazo[3,4-a]pyridine (29 g., Rf 0.25on thin layer chromatography on silica gel with ethyl acetate/5% aceticacid as eluant).

PREPARATION 7 Methyl 3-Benzoylacrylate

3-Benzoylacrylic acid (3 g.) was refluxed for 2 hours with 100 ml. ofmethylene chloride and 10 ml. thionyl chloride. Concentration to drynessgave 3-benzoylacrylyl chloride. Methanol (15 ml.) was added and theresulting solution stirred for 0.5 hour. Ether (35 ml.) was added. Theresulting organic mixture was washed in sequence with 15 ml. of water,15 ml. of 1N sodium hydroxide and twice with 15 ml. of water,concentrated to an oil, the oil triturated with 25 ml. of hot hexane,decanted from insolubles and the hexane concentrated to yield methyl3-benzoylacrylate (1.9 g., oil, m/e 190).

By the same procedure, 3-(4-methoxybenzoyl)acrylic acid3-(4-methylbenzoyl)acrylic acid, 3-(4-phenylbenzoyl)acrylic acid and3-(3-chlorobenzoyl)acrylic acid are reacted with sulfonyl chloride andthen methanol to form, respectively:

Methyl 3-(4-methoxybenzoyl)acrylate;

Methyl 3-(4-methylbenzoyl)acrylate;

Methyl 3-(4-phenylbenzoyl)acrylate;

Methyl 3-(3-chlorophenylbenzoyl)acrylate; and

Methyl 3-(cyclohexylcarbonyl)acrylate.

Those acylacrylic acids not available commercially are prepared byacylation of the appropriate benzene derivative with maleic anhydride[see Grummitt et al. Org. Syn. III, 109 (1955)], condensation of theappropriate methyl ketone with chloral, followed by hydrolysis anddehydration [see Koenigs and Wagstaffe, Ber 26, 558 (1893)], orhalogenation of the appropriate 3-acylpropionic acid followed bydehydrohalogenation [see Bougault, Ann. Chim. Phys. 15, 491 (1908)].

PREPARATION 8 4-Chlorophenoxyacetaldehyde Diethylacetal

Potassium hydroxide (56.1 g., 1 mole) was added slowly to 4-chlorophenol(128.5 g., 1 mole) and the mixture heated to obtain a solution.Chloroacetaldehyde (300 g.) was added over 30 minutes from a droppingfunnel and the mixture refluxed for approximately 16 hours (temperatureapproximately 175° C.). The reaction mixture was cooled to roomtemperature, diluted with approximately 300 ml. of water, productextracted into ether (three 200 ml. portions), the ether dried overanhydrous sodium sulfate, and the ether evaporated to an oil.Distillation of the oil afforded purified 4-chlorophenoxyacetaldehydediethylacetal (144.8 g., b.p. 158°-163° C./20 mm.)

PREPARATION 9 2-(4-Chlorophenoxy)-3-dimethylaminoacrylaldehyde

Dimethylformamide (109.5 g., 1.5 moles) was cooled in an ice-water bath.Phosphorus oxychloride (229.5 g., 1.5 moles) was added dropwise over 1.5hours and the reaction mixture then allowed to stir for 45 minutes atroom temperature. Chloroform (300 ml.) was added and then4-chlorophenoxyacetaldehyde dimethylacetal (122 g., 0.5 moles) and themixture refluxed for approximately 16 hours. The reaction was cooled toroom temperature and added slowly to approximately 300 ml. of chloroformmixed with ice and water while maintaining the pH approximately 10 withsodium hydroxide. Aqueous 40% dimethylamine (400 ml.) was added, theorganic layer separated and the aqueous extracted with additionalchloroform. The chloroform extracts were combined, dried over anhydroussodium sulfate, concentrated to a semisolid, and crystalline productrecovered by trituration with isopropyl ether (95 g., Rf 0.25 on thinlayer chromatography on silica gel with ethyl acetate as eluant).

PREPARATION 10 2-(4-Chlorophenoxy)-3-carbethoxymethylaminoacrylaldehyde

2-(4-Chlorophenoxy)-3-dimethylaminoacrylaldehyde (11.3 g., 50 mmoles)was dissolved in 200 ml. of ethanol, mixed with a solution of ethylglycinate (14 g., 100 mmoles) in 90 ml. of 1N sodium hydroxide andrefluxed for 15 hours. Ethanol was evaporated in vacuo, the aqueousresidue diluted with 150 ml. of water and product extracted into 200 ml.of ethyl acetate. The ethyl acetate was back-washed with water andconcentrated to an oil. Trituration of the oil with 100 ml. of ethergave crystalline2-(4-chlorophenoxy)-3-carbethoxymethylaminoacrylaldehyde in two crops(7.3 g., Rf 0.55 on silica gel thin layer chromatography with ethylacetate-1/hexane-1/5% acetic acid as eluant).

PREPARATION 11 2-(4-Chlorophenyl)-3-dimethylaminoacrylaldehyde

Phosphorus oxychloride (218 g., 2.18 moles) was added dropwise todimethylformamide (157 ml., 2.28 moles), maintaining the temperature at25° C. 4-Chlorophenylacetic acid (130.6 g., 0.76 mole), dissolved in amixture of 400 ml. of chloroform and 25 ml of dimethylformamide, wasadded in a stream and the reaction refluxed for approximately 16 hours.The reaction was cooled to room temperature and poured slowly into iceand water, while maintaining the pH approximately 10 with sodiumhydroxide. Dimethylamine (500 ml. of 25% aqueous) was then added and themixture heated on the steam bath for 1 hour. The mixture was cooled,extracted with four 500 ml. portions of chloroform, the chloroformextracts combined, dried over anhydrous sodium sulfate, treated withactivated carbon and evaporated to an oil. Trituration with coldisopropyl ether gave crystalline2-(4-chlorophenyl)-3-dimethylaminoacrylaldehyde (111 g., Rf 0.25 onsilica gel thin layer chromatography with ethyl acetate as eluant).

PREPARATION 12 2-(4-Chlorophenyl)-3-carbethoxymethylaminoacrylaldehyde

2-(4-Chlorophenyl)-3-dimethylaminoacrylaldehyde (90.2 g., 0.43 mole) wasdissolved in 1500 ml. of ethanol. Ethyl glycinate hydrochloride (60.2g., 0.86 mole) was dissolved in 900 ml. of water and the pH adjusted to7.5 with 6N sodium hydroxide. The solutions were combined and refluxedfor 6 hours. The ethanol was removed by evaporation, the productextracted into chloroform, the chloroform dried over anhydrous sodiumsulfate, evaporated to dryness, the solid residue triturated with etherand crystalline 2-(4-chlorophenyl)-3-carbethoxymethylaminoacrylaldehyde(73.6 g., m.p. 84°-86° C.) recovered by filtration.

I claim:
 1. A method of lowering the level of blood glucose in ahyperglycemic mammal which comprises administering to said hyperglycemicmammal a compound selected from the group consisting of pyrrolecarboxylic acids having the formula ##STR6## wherein A is selected fromthe group consisting of hydrogen and (C₁ -C₂)alkyl;R² is selected fromthe group consisting of benzyl, phenyl, chloro, 4-chlorophenyl and4-chlorophenoxy; and R³ is selected from the group consisting of benzyland chloro;or a pharmaceutically-acceptable salt thereof in an amountsufficient to lower said blood glucose level in said hyperglycemicmammal.
 2. A method of lowering the level of blood glucose in ahyperglycemic mammal which comprises administering to said hyperglycemicmammal a compound selected from the group of pyrrole carboxylic acidshaving the formula ##STR7## wherein A is selected from the groupconsisting of hydrogen and (C₁ -C₂)alkyl;R² is selected from the groupconsisting of benzyl, phenyl, chloro, 4-chlorophenyl and4-chlorophenoxy;or a pharmaceutically-acceptable salt thereof in anamount sufficient to lower said blood glucose level in saidhyperglycemic mammal.
 3. A method of claim 1 with a compound having theformula (VII).
 4. A method of claim 3 wherein A is hydrogen.
 5. Themethod of claim 2 wherein R² is 4-chlorophenyl.
 6. The method of claim 2wherein R² is phenyl.
 7. The method of claim 4 wherein R² is4-chlorophenoxy.
 8. A method of claim 1 with a compound having theformula (VIII).
 9. A method of claim 8 wherein A is hydrogen.
 10. Themethod of claim 9 wherein R³ is benzyl.
 11. The method of claim 9wherein R³ is chloro.